(3) The main steps of the experiment to verify urine sugar are as follows: ① Take two test tubes, numbered A and B respectively. Add 2ml of fasting urine of diabetics to A, and the same amount of fasting urine of healthy people to B. ② Take another test tube, add the same amount of solution A and solution B of Phyllin reagent to the test tube, and mix well. ③ Add the newly prepared Phyllin reagent 1ml into two test tubes A and B respectively.
(4) The experimental results produced in the above experimental process are as follows: Brick red precipitate appears in the test tube; B there is no brick red precipitate in the test tube.
(5) The main chemical reagents of biuret reagent and Linfei reagent are sodium hydroxide and copper sulfate. Therefore, in the absence of biuret reagent, if protein in urine of diabetic patients is detected, a certain amount of Linfei reagent B in step (2) can be diluted to 0.00 1ml, and in step (3), Linfei reagent A of 1ml is added first, and then it is added dropwise after shaking well.
So the answer is:
(2) The diabetic patient has diabetes on an empty stomach, and reacts with Phyllin reagent under the heating condition of 50-65℃ water bath to generate brick red precipitate; Healthy people have no diabetes, and there is no corresponding reaction on an empty stomach.
(3)① Equal amount of healthy people on an empty stomach ② Equal amount of igniters A and B, and mix them evenly.
(3) Put the newly prepared phenanthrene reagent 1 ml in warm water at 50-65℃ for 2 minutes.
(4) Brick red precipitate appears, but brick red precipitate does not appear.
(5) Dilute a certain amount of solution of burning reagent B to 0.00 1 ml, first add 1 ml solution of burning reagent A, shake it evenly, and then drop it respectively.