Chinese name: fibrinolytic enzyme. : 900 1-90-5 storage conditions:? Brief introduction, basic information, physical and chemical properties, medical examination, classification, principle, reagents, operation methods, normal values, clinical significance, related drugs, classification, pharmacological effects, indications, contraindications, adverse reactions, usage and dosage, expert comments, other research reports, and basic information of compounds Chinese name: fibrinolytic enzyme Chinese synonym: fibrinolytic enzyme; Fibrinolytic enzyme; Fibrinolytic enzyme; Human fibrinolytic enzyme; Human fibrinolytic enzyme; Fibrinolytic enzyme comes from human plasma. English name: fibrinolytic enzyme English synonym: Actasee.c.3.4.4.14; Fibrinoproteinase; Fibrinolytic enzyme (human); Serum tryptase; Thrombolysin; EC 3.4 . 2 1.7； Fibrinolytic enzyme CASNo : 900 1-90-5 Physical and chemical properties Storage conditions:? 20℃ form: freeze-dried powder medical examination classification blood biochemical examination >; Enzymatic determination principle Fibrin can combine with Congo Red to form a water-insoluble complex. Fibrinolytic enzyme in plasma can hydrolyze fibrin and release Congo red. Congo red is blue under acidic conditions, and its color is positively correlated with plasma fibrinolytic enzyme activity. Reagent (1) boric acid buffer (pH7.8): boric acid 6. 184g, KOH 7.456g, distilled water 500ml, 0. 1mmol/L NaOH solution 53ml, and add distilled water to 1000ml. (2) 1g/L Congo red solution: Congo red 0.5g, physiological saline 500ml. (3) 1mol/L hydrochloric acid solution. (4) 50g/L calcium chloride solution. (5) Preparation of matrix powder: the plasma, physiological saline and 50g/L CaCl2 solution are evenly mixed according to the ratio of 65,438+00: 65,438+000: 5, bathed in water at 37℃ for 60min, completely spread out the fibrin with a glass rod, washed twice with physiological saline, squeezed dry and dried in an incubator at 56℃. Take 10g fibrin, add 10g/L Congo red solution 100ml, stir in a water bath at 37℃, keep the temperature for 30min, centrifuge at 3000r/min10min, discard the supernatant, and repeatedly wash with boric acid buffer with pH7.8 until the supernatant. (6) spectrophotometer. Operation method (1) According to the table 1 Operation: (2) Centrifuge the above solution, take the supernatant, compare the color with a spectrophotometer at 600nm, adjust the blank to 0, determine its absorbance value, and check the standard curve to get the fibrinolytic enzyme activity. Note: Anticoagulant drugs, such as aspirin, are prohibited before the test. Normal fibrinolytic enzyme activity: 85.55% 27.83% (chromogenic substrate method) 2 1. 1 ~ 48.9 U (Congo red chromogenic method) Clinical significance (1) Effect of exercise on fibrinolytic system: Weiss and others discussed the relationship between exercise degree (moderate or severe) and coagulation and fibrinolysis system. The results showed that t-PA antigen increased from 3.7±0.5 ng/ml to 65438±0.8 ng/ml during moderate exercise, P