What is the principle of pipette? Why is it so accurate? What factors will affect its effectiveness?

There is a piston inside the pipette. Use your hands to control the movement of the piston to expel part of the air, insert the tip under the liquid surface, and then return the piston to its original position. In this way, under the action of the pressure difference, The liquid can be sucked into the suction head, and then press the piston to discharge the liquid. The accuracy of a pipette mainly depends on the sealing of the piston, the sealing of the tip and the pipette, and the accuracy of the piston control. There are many factors that affect the pipetting effect, such as human error, bad operating habits, operating environmental conditions, inappropriate operating methods, special liquid properties, pipette status, matching of the tip and pipette, etc. wait. Therefore, when choosing pipettes and tips, you must consider all aspects. Try to choose original pipette tips, and at least choose tips that have a good seal with the pipette. These are what Sartorius’s teacher said when he came to our pipetting academy last time. I think they are quite practical. They also brought a pipette to show that day. It felt great. I tested it on a scale and the accuracy was also very good!