Molecular biology technology platform to use what instruments and equipment

SICOLAB organized the molecular biology technology platform commonly used equipment are as follows:

(a) ordinary PCR instrument

Application:Widely used in various fields of molecular biology. Including gene diagnosis, isolation, cloning, nucleic acid sequence analysis, mutant and recombinant building blocks and gene expression regulation studies, such as Bio-RadS1000PCR instrument.

(II) Gradient PCR instrument

Application: for gradient PCR experiments. It is widely used in the optimization of PCR conditions, and can be used to set up 12 temperature gradients for the same batch of PCR amplification.

(C) Fluorescence quantitative PCR instrument

Application scope: for gene expression analysis (fluorescence quantitative PCR) and gene mutation (including HRM technology) detection.

(4)Ultra-micro UV-visible spectrophotometer

Scope of application: the use of spectrophotometry for quantitative and qualitative analysis of substances, mainly used for the detection of nucleic acid packages to assess the dsDNA, ssDNA, RNA and purified proteins, including BSA, IgG, lysozyme and other proteins of high purity.

(E)Pulsed field gel electrophoresis system

Application scope:Widely used in the molecular epidemiological research of many strains of bacteria. It can be used to analyze the phase identity between strains, assist in tracing the source of infection, and can play an important role in epidemic control.

(F) Nucleic Acid Gel Imaging System

Application scope:Used for the imaging analysis of electrophoresis products, can be used to analyze and image the electrophoresis products of different dyes, and at the same time, it has the functions of lane analysis, DNA molecular weight calculation, but one to be analyzed Dot-blot electrophoresis analysis and colony counting, etc. It can be used to analyze the molecular epidemiology of many strains of bacteria. It is included in Bio-RadEZ gel imaging system, ChemiDocXRS+ gel imaging system, GeldocXR+ gel imaging system and so on.

(VII) Fully Automatic Nucleic Acid Extraction Chest

Application: The fully automatic magnetic separation method is used to separate and purify the pathogen nucleic acids in the samples, which can be directly detected by hand PCR.

(viii) Roche 454 high-throughput sequencing platform

Application scope: for genome devo sequencing, transcriptome devo testing, macro genome sequencing, resequencing, etc..