PurposeEnsure the reliability of the autoclave effect. Scope of application Steam type autoclave. This SOP Change Procedure Changes to this standard operating procedure may be initiated by any staff member using this SOP and submitted for approval and signature by the following: professional supervisor, section chief. Operation method 1. Add water into the autoclave to its scale line, put the sterilized items into the autoclave, close the door of the autoclave, tighten the screws and make sure that it has been closed. 2. Open the exhaust valve. 3. Open the steam switch, input steam into the autoclave or connect the power supply to produce steam. 4. Observe the exhaust of the exhaust valve, and wait for the exhaust to be changed from cold gas to steam, and when the pressure gauge reaches 0.05 mPa, close the exhaust valve. 5. Observe the pressure gauge, and when it reaches 0.05 mPa, close the exhaust valve. 6. 5. Observe the pressure gauge, when the pressure rises to 0.15mPa, start timing. 6. Pressure up to 0.15mPa, you can adjust the inlet valve, reduce the amount of air intake, maintain pressure and make it stable at 0.15mPa. electric heating, you can cut off the power supply to maintain the pressure lasts for 15 minutes, up to no more than 30 minutes, otherwise the nutrients are destroyed. 7. Close the inlet valve or cut off the power supply, so that the pan Close the air inlet valve or cut off the power, let the pot cool down naturally, do not open the exhaust valve immediately, in order to avoid accidents. 8. When the pressure in the pot drops to zero, you can open the lid and take out the items. 9.
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