2.DNA desaturation Under the action of physical and chemical factors, the two complementary strands of DNA double helix become loose and become single strands, which leads to changes in the physical and chemical properties and biological properties of DNA. This phenomenon is called DNA degeneration.
3. Renaturation of DNA: A process in which denatured DNA can recombine two separate strands into a double helix structure under appropriate conditions.
4. Melting temperature (TM): the temperature corresponding to the midpoint of the increase of ultraviolet absorption in DNA thermal denaturation. Or the thermal melting temperature.
5. Coloring effect: When DNA denatures, the absorption of 260nm ultraviolet light increases.
6. hypochromic effect: With the renaturation of nucleic acid, the ultraviolet absorption decreases.
7. Nucleic acid exonuclease: An enzyme in ribonuclease and deoxyribonuclease, which can hydrolyze phosphodiester bonds in nucleic acid molecules.
8. Nucleic acid exonuclease: An enzyme that hydrolyzes nucleotides one by one from one end of a nucleic acid chain.