1. High-pressure steam source: A stable high-pressure steam source is needed, usually generated by a steam generator or pressure cooker. The pressure of steam must be able to meet the specific sterilization requirements.
2. High temperature: The high-pressure steam sterilization method needs to raise the temperature to above about 12 1 degree Celsius (250 degrees Fahrenheit). This temperature can be reached and maintained for a period of time in a high pressure environment.
3. Pressure vessels: High-pressure vessels are used to hold things. The container must be able to withstand the pressure of high-pressure steam and remain sealed to ensure the effectiveness of the sterilization process.
4. Appropriate time: High pressure steam sterilization needs to be carried out within a specific time range to ensure that the target microorganisms are fully killed. According to different objects and requirements, sterilization time may be different.
5. Preparation of target: The target after sterilization must be properly cleaned and prepared in advance to ensure the sterilization effect. Anything that may prevent steam from reaching the target area should be removed.
6. Adjustment and monitoring: The high-pressure steam sterilization process needs accurate adjustment and monitoring to ensure proper temperature, pressure and time, so as to achieve the effect of complete sterilization.
Effect of high pressure steam sterilization
High-pressure steam sterilization can effectively kill all kinds of microorganisms and ensure the sterility and safety of products and equipment through the action of high-temperature and high-pressure steam. This is a widely used and effective sterilization method.
1. Killing microorganisms: autoclaving can completely kill bacteria, viruses, fungi and other microorganisms, including pathogenic bacteria, at high temperature and high pressure. High temperature and high pressure can destroy the cell wall, cell membrane and nucleic acid of microorganisms, thus inactivating them.
2. Efficient sterilization: high-pressure steam sterilization is fast and efficient, and can achieve sterilization effect in a short time. Usually, the high temperature and high pressure of about 12 1 degree Celsius (250 degrees Fahrenheit) need to be maintained for a period of time to ensure the complete killing of microorganisms.
3. Wide application: High pressure steam sterilization can be used to sterilize many different types of objects and equipment, including medical instruments, laboratory instruments, medicines and food containers. Widely used in medical, pharmaceutical, food processing and other fields to ensure the sterility and safety of products.
4. No chemical residue: Compared with some chemical sterilization methods, the high-pressure steam sterilization method does not use chemical disinfectants, so it will not produce chemical residues. This makes it especially suitable for objects and environments sensitive to chemical substances.
5. The sterilization effect can be detected: the effect of high-pressure steam sterilization can be monitored and verified by sterilization indicator and culture medium inoculation. This can ensure the effectiveness and reliability of the sterilization process.