Specifically: after adding DNA, synthetic raw materials (dNTP, primers), and heat-resistant DNA polymerase (taq) into the EP tube, put it into the PCR instrument, which will use the elevated temperature to denature the DNA, and then make single-stranded replication into double-stranded replication under the action of the polymerase, which will lead to the purpose of gene replication.
Uses:
1, basic research on nucleic acids: genome cloning
2, asymmetric PCR to prepare single-stranded DNA for DNA sequencing
3, reverse PCR to determine the unknown DNA region
4, reverse transcription PCR (RT-PCR) for detecting the level of gene expression in the cell, the amount of RNA viruses and Direct cloning of cDNA for specific genes
5. Fluorescence quantitative PCR for real-time monitoring of PCR products
6. cDNA end rapid amplification technology
7. Detection of gene expression
8. Medical applications: detection of bacterial and viral diseases; diagnostics of genetic disorders; diagnostics of tumors; forensic forensic science