Disease material taken, such as can not be immediately tested, or need to be sent to the relevant units for testing, should be added to the appropriate amount of preservative disease material to keep as fresh as possible.
1. The preservation of bacteriological test material will be taken to the organ tissue block, preserved in saturated sodium chloride solution or 30% glycerol buffered saline solution, containers with plugs sealed. If the system is liquid, can be installed in a closed capillary glass tube or test tube transportation. Saturated sodium chloride solution preparation method is: distilled water l00ml, sodium chloride 38 ~ 39g, fully stirred to dissolve, filtered with several layers of gauze, autoclaved and ready to use. 30% glycerol buffer saline solution preparation method is: neutral glycerol 30ml, sodium chloride 0.5g, sodium alkaline phosphate 1.0g, add distilled water to 100ml, mixed with autoclaved and ready to use.
2. The preservation of viral test materials will be taken to the organ tissue block, preserved in 50% glycerol buffered saline solution or egg saline, containers with plugs sealed. 50% glycerol buffered saline solution is prepared as follows: sodium chloride 2.5g, 0.46g of acidic sodium phosphate, alkaline sodium phosphate 10.74g, dissolved in 100ml neutral distilled water, add pure neutral glycerol 150m1, neutral distilled water, neutral distilled water, and then mixed and autoclaved. 150m1, 50ml of neutral distilled water, mixed and divided, autoclaved standby. Egg saline preparation method is: the first fresh egg surface disinfection with iodine, then open the contents of pouring people sterilized containers, according to the whole egg 9 parts to add 1 part of sterilized saline, shaking well filtered with sterilized gauze, and then heated to 56 ~ 58 ℃ for 30min, the second and third days according to the above method to heat up again, can be applied.
3. The preservation of pathology and histology test materials will be taken to take the organ tissue block put in lo% formalin solution or 95% alcohol fixation; the amount of fixation solution should be more than 10 times the amount of material sent for examination. If fixed with 10% formalin solution, it should be changed to fresh solution once after 24h. In order to prevent the disease material from freezing in the cold season, you can take out the above fixed tissue blocks and save them in a mixture of glycerol and 10% formalin in equal quantities.