What is the δ rn value in quantitative pcr?

Δ rn = R+n-R-n, calculate Δ rn, where r+n represents the fluorescence intensity measured at each point and r-n represents the fluorescence baseline intensity.

Real-time fluorescence quantitative PCR uses the change of fluorescence signal to detect the change of amplification products in each cycle of PCR amplification reaction in real time, and quantitatively analyzes the initial template copy number in the sample through the analysis of cycle threshold and standard curve.

Extended data:

trait

Due to the application of fluorescent substances, real-time fluorescence quantitative PCR can directly detect the change of fluorescence signal during PCR amplification through photoelectric conduction system and obtain quantitative results, which overcomes many shortcomings of conventional PCR and has the following advantages:

(1) can quantify the template;

② Closed reaction, no post-treatment of PCR, less pollution and low false positive rate;

(3) automatic observation and recording, intuitive results, avoiding errors caused by human judgment;

(4) The working efficiency is high, which is beneficial to the detection of Qualcomm.

Shortcomings of real-time fluorescence quantitative PR;

(1) special equipment and fluorescent probes are needed, and the cost is high;

② The fragment length of PCR products could not be displayed;

③ Quantitative accuracy needs to be improved.

Baidu encyclopedia-quantitative PCR