A PCR laboratory layout
PCR laboratory in principle for the reagent preparation area, sample preparation area, amplification area and analysis of amplification products in four separate work areas and a dedicated corridor, the work area should be set up buffer room, work area and buffer room should be installed with a chain device. Different functions of the work area should be separated and independent, the work area has a clear sign, can not be straight through, if closely linked, need to install items transfer window. The first two areas are pre-amplification areas, the last two areas are post-amplification areas, and the pre-amplification areas and post-amplification areas should be strictly separated. Experimental materials, reagents, recording paper, pens, cleaning materials, etc., can only flow from the pre-amplification area to the post-amplification area, i.e., from the reagent preparation area → sample preparation area → amplification area → amplification product analysis area, and shall not flow in the reverse direction. Laboratory airflow should also flow from the pre-amplification area to the post-amplification area, not reverse flow. The top of the work area should be installed in the ultraviolet lamp, ultraviolet lamp wavelength of 254nm, every 20 square meters of a 40W ultraviolet lamp.
Two, the function of each area of the laboratory and the main equipment
1, reagent preparation area: mainly for the preparation of reagents, dispensing and preparation of the main reaction mixture. Reagents and materials used for sample production should be transported directly to the area, not through other areas. Reagent raw materials must be stored in this area and prepared into the required reagents in this area. The main equipment in this area includes balance, refrigerator, centrifuge, sample filler, oscillator and so on.
There are no strict requirements for the control of airflow pressure in this zone.
2, sample preparation area: mainly for the preservation of samples, nucleic acid (RNA, DNA) extraction, storage and its addition to the amplification reaction tube and determination of DNA synthesis. The main equipment in this area includes refrigerator, biological safety cabinet, centrifuge, sample adding device, oscillator, constant temperature water bath and so on.
The pressure gradient in this area is required to be positive relative to neighboring areas to avoid aerosol contamination from neighboring areas into this area.
3, amplification zone: mainly for DNA amplification. In addition, the DNA template (from the sample preparation area) and the main reaction mixture (from the reagent preparation area) can be prepared into a reaction mixture, etc. can also be carried out in this area. The main equipments in this area are: amplifier, refrigerator, centrifuge, and sample spiker.
The pressure gradient in this area is required to be negative relative to neighboring areas to avoid aerosol leakage from this area.
4, amplification product analysis area: the determination of amplification products. The main equipment in this area are enzyme labeling instrument, plate washer, sample filler and water bath box.
The pressure gradient in this area is required: negative pressure relative to the neighboring areas to avoid the amplification products from this area to spread to other areas.
Three, PCR laboratory ventilation system