Goods No.: F007 Prothrombin Time (PT) test kit (2ml * 10 bottles) 360.00 yuan
There are also imported. For example, Human Prothrombin,PT ELISA Kit, RB's, Abnova's. These have to be purchased on behalf of the biotech company.
Another solution is to get a biotech company to do it for you, which is also possible.
Product Name Prothrombin Time Assay Kit (Coagulation Method)
Package Specification PT Reagent: 2.0ml×10; PT Reagent Dissolution: 5.0ml×4
Intended Use This kit is used for in vitro assay of human plasma plasminogen time (PT) to qualitatively screen the condition of exogenous coagulation system.
Main components PT reagent: rabbit brain powder saline extract (lyophilized product); PT reagent dissolution solution: calcium chloride solution. Storage conditions and expiration date 1.The kit is valid for 12 months at 2℃～8℃; 2.The reagent for compound dissolution is valid for 7 days at 2℃～8℃; 3.The reagent for compound dissolution is stable at 37℃ for 8 hours.
Applicable instruments Manual method or semi-automatic blood coagulation analyzer.
Sample requirements Intravenous blood collection, 1 part of anticoagulant (0.109 mol / L trisodium citrate) collection of 9 parts of whole blood , gently inverted and mixed, centrifuged at 2700g (centrifugal radius of 18cm about 3500 rpm) for 10 minutes, separated plasma to be measured. If the plasma can not be analyzed immediately, it should be sealed and stored at 2 ℃ ~ 8 ℃, the longest time not more than 4 hours; or immediately placed in -20 ℃ below the freezing not more than one month; -70 ℃ below the freezing, not more than half a year, with the use of 37 ℃ thawing, to avoid repeated freezing and thawing.
Test Methods Reagent Preparation: 1. accurately add 2.0ml PT reagent dissolution solution to each bottle of PT reagent, shake gently to dissolve; 2. take the appropriate amount of re-solubilized PT reagent, and keep warm at 37℃. Experimental steps: 1. Take plasma to be tested or normal coagulation quality control plasma ※ 0.1 ml, 37 ℃ heat preservation for 3 minutes; 2. Rapidly add 0.2 ml has been pre-warmed to 37 ℃ PT reagent, mixing, and immediately timed, recorded its coagulation time, that is, the prothrombin time (PT). If the blood coagulation analyzer is used for determination, please refer to the instruction manual of the instrument. ※ This normal coagulation quality control plasma is not included in this kit. Calculation of results: 1. Prothrombin time in seconds (PTs): i.e., the measured coagulation time (PTs of normal or abnormal plasma samples) PTs of the examined plasma 2. PT ratio (PTR) = ────────── PTs of normal coagulation quality-control plasma 3. International normalized ratio (INR) = PTRISI
Reference range 1. Expressed as PTs value: 10 to 15 seconds. 2. Expressed as PTR: 0.95 to 1.24. 3 Monitoring oral anticoagulants, expressed as INR. Note: The above values are for reference only. It is recommended that each laboratory establish its own range of reference values. New reference ranges should be re-established whenever reagents, instrument operation, blood sample collection techniques or anticoagulants are changed.
Interpretation of test results 1. Comparison with normal coagulation quality control plasma PTs value, more than 3 seconds is abnormal. 2. PTR value exceeding the range of 0.95 to 1.24 is considered abnormal. 3. Monitor oral anticoagulants, expressed as INR.
Precautions 1. This product is for in vitro diagnosis only. 2. Blood should be drawn smoothly and anticoagulated sufficiently without clots; when separating plasma, make sure to remove platelets. 3. Keeping temperature is controlled at 37±0.5℃, too high or too low will affect the results. 4. EDTA, heparin or oxalate should not be used as anticoagulant during sample collection. 5. The light for observing the coagulation should be sufficient for manual determination. 6. The ratio of anticoagulant should be accurate. If the hematocrit is less than 20% or more than 55%, the ratio of blood sample to anticoagulant needs to be adjusted by: 0.00185 x ml of whole blood x (100 - hematocrit) = ml of anticoagulant. 7. Prevent the reagent from being contaminated by any trace amount of plasma. 8. Do not test sample plasma of doubtful quality. This is because very small plasma coagulation will result in a significant reduction in clotting time (all clotting factors have been activated), and extensive coagulation will in turn prolong the clotting time due to depletion of clotting factors and fibrinogen. 9. The presence of a thrombin inhibitor in a sample may result in a prolonged prothrombin time for that sample. 10. Users who inadvertently splash reagents on the skin or in the eyes should rinse immediately with water.