What is the difference between the ultra-clean bench and the biological safety cabinet?

1 Biological safety cabinet is generally do pathogenic bacteria, mold yeast to use, the operating area is negative pressure, simply put is to draw wind, of course, pathogenic bacteria is not directly out of the exhaust to the outdoors, is filtered in the top of the biosafety cabinet leakage, from the amount of exhaust can be divided into 50% of the external discharge type, 75% and 100% of the external discharge type.

2 Ultra-clean table is to do general no direct harm to the human body of routine bacterial operation experiments, positive pressure air supply, blowing to the samples and the experimental staff of the wind is filtered through the filter, to achieve the local level 100.

3 Ultra-clean bench is equivalent to a reduced sterile room (sterile space) Detailed description is as follows: biological safety cabinet and ultra-clean bench difference is as follows: biological safety cabinet is for the operation of primary cultures, strains of bacteria and diagnostic specimens and other infectious experimental materials, used to protect the operator himself, the laboratory environment and experimental materials, so that it avoids exposure to infectious aerosols and other materials that may be generated in the course of the above operations. It is designed to protect the operator, laboratory environment and laboratory materials from exposure to infectious aerosols and spills that may be generated during these operations. The WHO Laboratory Biosafety Manual states that biosafety cabinets are effective in minimizing laboratory infections and cross contamination of cultures due to aerosol exposure. Biosafety cabinets also protect the work environment. In fact, biosafety cabinets are more focused on protecting operators and the environment from the spread of pathogenic microorganisms from operations that can cause injury to personnel and contamination of the environment. According to the difference in the level of biosafety protection, biosafety cabinets can be categorized into three types: Class I, Class II and Class III. Class I biosafety cabinets protect staff and the environment but not samples. The principle of airflow is basically the same as that of a laboratory fume hood, except that a HEPA filter is installed at the exhaust port to filter the external airflow and thus prevent the spread of microbial aerosols that can cause contamination. Class I biosafety cabinets do not have a fan and rely on an external fan in the fume hood to drive the airflow, which is less commonly used nowadays as it does not protect the products inside the cabinet. Secondary biological safety cabinet is currently the most widely used cabinet type. In accordance with the provisions of the "Chinese People's Republic of China * * * and the State Pharmaceutical Industry Standard YY0569-2005 biological safety cabinets", the two biological safety cabinets in accordance with the inlet air velocity, exhaust and circulation can be divided into four levels: A1-type, A2-type, B1-type and B2-type. All Level II Biosafety Cabinets provide protection for staff, the environment and products. The advantages of an ultra-clean bench are ease of operation, comfort, high efficiency, short set-up times, and essentially ready-to-use. This is a general-purpose local purification equipment for single-operator, the airflow form of vertical laminar flow and horizontal laminar flow, which can create a local high-cleanliness air environment, is the scientific research and pharmaceutical, medical and health care, electronics and optical instruments, and other industries, the most ideal special equipment. In the factory production, inoculation workload is very large, need to often work for a long time, the ultra-clean table is very ideal equipment. Ultra-clean table by the three-phase motor for the wind power, power 145 ~ 260W or so, the air through the special microporous foam layer laminated by the composition of the "super filter" blown out of the formation of a continuous dust-free and sterile ultra-clean air laminar flow, that is, the so-called "high-efficiency special air", it removes the dust, bacteria-free air, which is the most ideal special equipment for medical and health care, electronics and optical instruments and other industries. The so-called "high-efficiency special air" removes dust, fungi, and bacterial spores larger than 0.3 μm. The flow rate of the ultra-clean air is 24 to 30 m/min, which is sufficient to prevent contamination caused by the possible intrusion of nearby air, and such a flow rate does not prevent the use of alcohol lamps or Bunsen burners for sterilizing instruments, etc. The staff members are kept in such sterile conditions. The staff operates under such aseptic conditions, keeping the sterile material free from contamination during the transfer and inoculation process. However, in the event of a power failure in the middle of an operation, it is difficult to avoid contamination of materials exposed to unfiltered air. In this case, the work should be finished quickly and the bottle should be marked. If the material in the bottle is in the proliferation stage, it should not be used for proliferation in the future and should be transferred to the rooting culture. If the material is in the general production stage, it can be discarded because it is extremely abundant. If it is in the rooting process, it can be kept for future planting.