Sample biology lab report format Article 1
Experiment name: Observation of chloroplasts and cytoplasmic flow with a high-magnification microscope
I. Experiment purpose
1. Preliminary mastery of the use of high-magnification microscope.
2. To observe the morphology and distribution of chloroplasts in the cytoplasmic matrix of higher plants
2. Experimental principles
The chloroplasts of higher plants are ellipsoidal, and under different light conditions, the chloroplasts can be moved to change the direction of the ellipsoid, so that it can receive more light and not be burned by the strong light. In the strong light, the chloroplast to its ellipsoid side toward the light source; in the low light, the chloroplast to its ellipsoid front toward the light source. Therefore, the shape of the chloroplast ellipsoid in the leaflets of cucurbit moss collected under different light conditions is not exactly the same.
The cytoplasm in living cells is in a constant state of flow, and the movement of chloroplasts in the cytoplasmic matrix can be used as a marker to observe the flow of cytoplasm.
Three, materials and appliances
Moss leaves, fresh black algae, microscope, slides, coverslips, droppers, tweezers, razor blades, Petri dishes, pencils
Four, experimental procedures see book p30
1. make moss leaves of the fashion film
2. Fashion slides
4. Observing cytoplasmic flow with a microscope
V. Discussion
1. Are the chloroplasts in the cytoplasmic matrix stationary and why?
2. What is the relationship between the morphology and distribution of chloroplasts and the function of chloroplasts?
3. The cytoplasm of a plant cell is in a constant state of flow, what is the significance of this for the completion of life activities in living cells?
4. Draw a pencil drawing of a leaf cell, labeling the approximate direction of chloroplast flow.
Sample Biology Lab Report Format Article 2
Experiment Identification of Reducing Sugars, Lipids, and Proteins in Biological Tissues
I. Objectives of the Experiment
Preliminarily to master the basic methods of identifying reducing sugars, lipids, and proteins in biological tissues.
II. Experimental principles
1. Principle of identification of reducing sugars There are many types of reducing sugars prevalent in biological tissues, the common ones are glucose, fructose, maltose. Their molecules contain reducing groups free aldehyde group or free ketone group, so it is called reducing sugar. Sucrose has no free hemiacetal hydroxyl group in its molecule, so it is called a non-reducing sugar and does not have reducing properties. In this experiment, the Ferrin reagent can only be used to test for the presence or absence of reducing sugars in biological tissues, but not to identify non-reducing sugars.
Ferrin's reagent is prepared from a sodium hydroxide solution with a mass concentration of 0.1 g/ml and a copper sulfate solution with a mass concentration of 0.05 g/ml, which, when mixed, immediately produces a light blue precipitate of cuoh2. cuoh2 is able to produce a brick-red precipitate of cu2o with glucose added to it under heating conditions, which is itself oxidized to gluconic acid. The reaction formula is as follows:
ch2oh-choh4-cho+2cuoh2→ch2oh-choh4-cooh+cu2o↓+2h2o
When reducing sugar is identified with Ferrin's reagent The color of the solution changes as follows: light blue, brown, brick-red precipitate.
2. Principle of protein identification Identification of biological tissues contain proteins, commonly used in the bioconjugate method, the use of bioconjugate reagent. The components of the bioconjugate reagent are sodium hydroxide solution with a mass concentration of 0.1 g/mla and copper sulfate solution with a mass concentration of 0.01 g/mlb. In the alkaline solution naoh, the bis-urea h2noc-nh-conh2 can interact with cu2+ to form a purple or purplish-red complex, a reaction called the bis-urea reaction. Since protein molecules contain many peptide bonds similar in structure to those of biuret, proteins can react with biuret reagents in color.
3. Principle of identification of fat Fat can be stained orange by Sudan Ⅲ and red by Sudan Ⅳ
Three, the experimental process see the book p18
Four, the experimental supplies see the book p18
Five, attention
1. On the identification of reducing sugars, the experiment, in the heating of the solution in the test tube, you should be used to test-tube clamps clamped to the upper part of the test tube, and put it into a large beaker of boiling water and heat it. Be careful that the bottom of the test tube does not touch the bottom of the beaker and that the mouth of the test tube does not face the experimenter, so that the solution in the test tube does not boil out of the test tube and cause burns. If the solution in the test tube boils too much, you can lift up the test tube clamp so that the bottom of the test tube leaves the boiling water in the large beaker.
2. Ferrin's reagent can only be used after the A and B solutions are mixed well, do not add the A and B solutions separately to the tissue sample solution.
3. In the identification of proteins, first add urea a, then add urea b
Six, discussion
What is the basis for the identification of reducing sugars, fats, and proteins in biological tissues?
Biology Lab Report Format Sample Essay 3
I. Objectives of the experiment
1. To observe the process of mitosis in plant cells and to identify the different periods of mitosis.
2. To acquire preliminary skills in making mitotic mounts of onion root tips.
3. Initial skills in drawing biological diagrams.
Two, the experimental principle
In the plant body, mitosis is common in the root tip, stem tip and other meristematic zone cells, and the process of mitosis in higher plant cells is divided into the interphase and the pre-division stage, middle stage, late stage, and the end of the division stage. You can use a high power microscope to observe the process of mitosis in plant cells, according to the changes in the chromosomes or chromatin in the cell during each period, identify which period of mitosis the cell is in, and the chromosomes in the nucleus of the cell can be easily colored by basic dyes.
Three, materials and utensils
Onion root tips, microscope, slides, coverslips, droppers, tweezers, Petri dishes, pencils, hydrochloric acid with a mass fraction of 15%, 95% alcohol by volume, and Gentian violet or violet water with a mass fraction of 0.01g/ml
Four, experimental procedures see book p39
1. 3-4 days in advance
2. Dissociation: 5min
3. Rinsing: 10min
4. Staining: 5min
5. Preparation
6. Microscopy
V. Note
1. Adequate dissociation is a necessary condition for the success of the experiment. Dissociation is sufficient for the tissue to be dispersed, and the cells will not overlap.
2 . The rinsing time must be sufficient, otherwise the cells will not stain.
3 . When staining, the concentration of the staining solution and the staining time must be mastered. In particular, the staining must not be too deep, otherwise a purple color under the mirror can not be observed.
VI. Discussion
1. What is the key to making a good mitotic mount of onion root tips? Talk about your own experience.
2. After observing the cells clearly at each period of mitosis, draw a sketch of mitosis in an onion root tip cell and label the periods.
Biology Laboratory Work Plan
The laboratory is the main place for students to learn and conduct experiments, the main resource for biological inquiry learning, and an important way for students to carry out scientific inquiry. Therefore, the school attaches great importance to the construction of the biology laboratory, configure the necessary instruments and devices to ensure that every student can carry out experimental inquiry activities, and create good conditions for students to carry out experimental inquiry activities. Through experiments, students can most effectively master the basic biological knowledge necessary for further study of modern science and technology, and cultivate preliminary practical skills and creative ability. Teaching focuses on cultivating students' scientific experimental ability and improving students' scientific experimental literacy, so that students can improve their self-learning ability, comprehensive analytical ability to use knowledge, hands-on ability and design and innovation ability while acquiring knowledge.
I. Guiding ideology
In line with the idea of serving students, vigorously cooperate with subject teachers to carry out experimental teaching, and cultivate students' skillful experimental operation skills.
Second, the key work
1, for the new curriculum teaching with new laboratory equipment.
2, to ensure that each experiment according to the requirements of quality and quantity in a timely manner.
3, with any subject teacher to do a good job of experiments in all years of students to strengthen textbook knowledge.
Three, specific work
1, 100% of the demonstration experiments, student experiments, and according to the requirements to ensure the quantity and quality of teachers in the classroom before the layout of each experiment, and never delay the impact of teaching.
2, on the experimental class, in the case of I have no class to go to the laboratory rounds, to help teachers troubleshooting, to solve the problem. Instruments are broken, reagents are not enough, repair and replenishment, guidance to help students correct the wrong operation method.
3, the experiment is completed, check the number and quality of instruments in a timely manner, such as errors in accordance with the system to deal with; timely replenishment of reagents to ensure the smooth progress of the next experiment; do a good job of the relevant experimental records, such as the time, the number of people, prone to failure and improve the method.
4, improve the management system, such as "laboratory, instrument room use of the management system", "laboratory safety code", "laboratory glass breakage compensation regulations" and so on, and on the wall. Frequent cleaning, so that the instrument dust-free, clean classroom.
5, the beginning of the period, the end of each account for a proofreading, so that the two are consistent, and do a good job of the relevant records of damage. Usually often review the experimental equipment and laboratory supplies, can be repaired in a timely manner repair, insufficient timely purchase.
6, supplement the new curriculum teaching laboratory equipment.
Four, strengthen the safety awareness, to ensure laboratory safety
To ensure laboratory safety, clear laboratory duties, regular inspection of fire extinguishing equipment and other devices, the establishment of the management of the person responsible for self-checking, the laboratory organization of the random safety inspection system. Strengthen the safety awareness.
To the laboratory safety responsibility of the main, experimental instructors with the cooperation of the Department of leadership care support, student cooperation, to ensure that the laboratory throughout the year without all kinds of safety accidents.