1, heating exhaust
Open the exhaust valve, so that the water boiling to remove the cold air inside the pot. When the cold air is completely exhausted, close the exhaust valve.
2, keep warm and maintain pressure
When the pressure in the pot rises to 1Kg/cm2, control the heat source and maintain the pressure for 15 minutes.
3, out of the pot
Shut off the power supply, let the temperature inside the sterilization pot naturally drop, when the pressure drops to 0, open the exhaust valve, loosen the bolts, open the lid, take out the sterilized items. Otherwise it will be due to the high pressure in the pot, the pressure difference caused by opening the air valve may make the culture medium in the container splash caused by cotton plugs stained with culture medium and contamination, or even make the container explode.
4, processing
After sterilization, the experimental equipment is usually put into the 60 ℃ ~ 80 ℃ oven drying, in order to remove the moisture of the sterilization, to avoid causing contamination.
Precautions:
1, items placed in the requirements of neat, stable. And need to leave a gap.
2, when adding the lid will cover the exhaust hose inserted into the inner sterilization barrel exhaust slot, in a two by two symmetrical way at the same time tighten the relative two bolts, so that the bolts tighten and loosen the same, do not make the leakage.
3. When opening the lid of the sterilization pot at the end of sterilization, be careful not to put your face close to it, otherwise the steam will cause damage to your face.