The inoculation room of edible fungi needs to be sterilized to better ensure the growth environment of edible fungi. The following is the method of disinfection and sterilization of edible mushroom inoculation room that I introduced to you, take a look at it.
Edible fungi inoculation room sterilization: ultra-clean bench Aseptic operating procedures
(1) Keep clean ultra-clean
Bench should be placed in a dry, clean room, and often keep clean. Each time before the use of inoculation materials and inoculation tools in an orderly manner on the bench, and check whether complete, and then UV irradiation for 30 minutes, sterilization.
(2) purify the operation
Before the inoculation of the machine, in order to purify the air in the operating area of the air environment, to be the normal operation of the machine 20 minutes, the operating area of the air purification, and then carry out inoculation.
(3) standardize the operation
Inoculation personnel wearing sterile overalls and shoes, wear masks and hats, inoculation equipment on both sides of the table, the operator's hands on the downwind side of the inoculation material. It is strictly prohibited to scratch the head when working, and strive to be agile when inoculating to minimize the chance of contamination, used matchsticks, cotton balls and pull out the old fungal skin into the container, shall not be discharged.
(4) inoculation inoculation is complete, will have been inoculated test tubes or bottles (bags) affixed with a good reprocessing labels, put into the culture room (box) for culture, with bactericidal solution to wipe clean the surface of the table and the ground.
(5) formaldehyde regular fumigation of the management program with the inoculation room, (box) processing.
Edible fungi inoculation room sterilization: inoculation room (box) aseptic operating procedures
(1) keep clean
Frequent cleaning, to keep the inoculation room (box) inside and outside clean.
(2) strict disinfection
Before use, test tubes or vials (bags) and inoculation tools in an orderly manner in the room (box) and on the bench, and check whether complete, hat and mask hanging in the buffer room hat hook, and then ultraviolet irradiation or disinfection of the drug fumigation.
1 hour later, the operator into the buffer room, wash their hands with soap and water, change clothes, shoes, masks, hats, into the inoculation room or put their hands into the inoculation box, and then sprayed with disinfectant king to reduce the dust and sterilization, ready for inoculation.
(3) standardize the operation
staff before inoculation with 75% alcohol cotton ball wipe hands, wipe the inoculation tool and bottle bag, to prevent cross-infection. Inoculation with an alcohol lamp burn inoculation tools, cotton plugs, test tube mouth, bottle (bag) mouth does not leave the flame area, such as cotton plugs accidentally on fire, covered with a wet cloth to allow its lack of oxygen to extinguish. Inoculation should be agile, cooperation should be tacit understanding, work is strictly prohibited scratching the head and other people in the room walking quickly to reduce the amount of dust. After the matchstick, cotton balls and pull out the old fungal skin should be put into the container, not put.
(4) post-inoculation treatment
Inoculation is complete, the inoculated tubes or bottles (bags) labeled, put into the culture room (box), culture, the waste out of the room (box) outside the incineration or buried, will be cleaned up the room (box).
(5) formaldehyde fumigation
Inoculation room (box) continuous inoculation using 10 days or half a month, the use of inoculation intervals, with formaldehyde fumigation, and then continue to use.