1, the United States UniBest two insects detection system, its sales in the global market ahead of other manufacturers.
2, the United States UniBest's two insects elution system, the use of new technology elution, through the natural elution mode of the collection of the filter capsule for the full amalgamation, not only 100% of the two insects amalgamated, but also to ensure the integrity of the two insects.
3. The novel and unique structure of the filter capsule ensures that the two worms are completely retained and easy to elute out, and it is more suitable for dealing with high turbidity water samples, which is in line with China's national conditions.
4. The design of disposable filter capsule avoids cross contamination and ensures the real validity of the two bugs experiment.
5, up to 24 months of reagent warranty period, not only to meet the customer's testing needs of the year, but also to ensure that the reagents are still effective in the next year's test, so that the reagents will be more maximize the use of reagents to avoid the short shelf life of reagents caused by the economic losses for the unit and the enterprise to save costs.
Second, the detection steps
GB 5750.12- 2006 "Standard Test Methods for Drinking Water" in the Giardia flagellates and Cryptosporidium
detection steps are as follows: sampling and filtration → elution and concentration → immunomagnetic separation → fluorescence staining → microscopic counting
Step 1: Sampling and filtration
Filtering and concentration → Immunomagnetic separation → fluorescence staining
The first step: sampling and filtration ?
Features of the filter capsule:
1, specially designed for the elution of the two worms, in line with the Chinese national standard GB
5749-2006 and the U.S. EPA, the British DWI, Australia TGA, and so on.
2. 0.45μm pore size, 100% retention of the two worms
3. Single-use to avoid cross-contamination
4. High-pressure resistance
Step 2: Elution and concentration
Features:
1. One-button full-automatic and rapid collection of the two worms samples for repeated elution.
2, the elution time is only 2 minutes, the whole experiment only 5 hours, without the need for late maintenance and repair, the world's lightweight and fast elution equipment, suitable for emergency and laboratory operations.
3, the equipment recovery rate of up to 90%, is currently the world's recovery rate of the upper limit of the two insects detection equipment.
Step 3: Immunomagnetic Separation
The immunomagnetic separation process uses UniBest's ClearScan series of immunomagnetic separation kits for the two worms, using magnetic beads with specific antibodies to combine with the Giardia and Cryptosporidium specific antigens in the samples, and efficiently separating them in a magnetic field, which ensures the specificity of the test. specificity.
Step 4: Fluorescence staining / microscopy
The stained samples were examined using a special fluorescence microscope for the two worms, and the samples were observed, and the observed samples that conformed to the size, shape, and fluorescence characteristics of sporocysts and oocysts were analyzed qualitatively and quantitatively in accordance with the characteristics of Giannoflagellate sporocysts and Cryptosporidium oocysts as described in the national standard GB/T 5750.12-2006.
Three, technical parameters
1, filtration step recovery ≥ 100%
2, filtration / elution step recovery ≥ 80%
3, IMS immunomagnetic separation step recovery ≥ 90%
4, the total recovery (filtration, elution, magnetic bead separation, staining) & gt; 35%
5, the detection of Relative standard deviation of the results ≤ 10%
6, elution pressure: 0.5bar, without compressed air can quickly complete the elution step, this advanced elution method, not only to ensure that the two worms are completely eluted, but also to ensure that the two worms intact form.
7, the test can deal with high turbidity water samples: >100 NTU
8, the kit is equipped with pre-made positive control slides, to ensure the effective activity of the kit; completely avoid the misdetection, to ensure the accuracy of the test.
9. Shelf life: the shelf life is up to 24 months, which is the longer shelf life and more stable performance of IFA reagents in the global industry.
10, the filter capsule can be recycled, high-pressure resistance
11, the water sample processing capacity: each time to deal with water ≥ 1000L tap water; ≥ 100L source water
12, the upper limit of the flow rate of the sampling: 4 liters / min
13, the total time of the elution step: 2 minutes
14, the structure of the filter module: including ≥ 100 porous Reticulated foam layer, two different specifications: ≥ 60 large foam layer, outer diameter 42mm, inner diameter 34mm; ≥ 40 small foam layer, outer diameter 30mm, inner diameter 25mm, thickness of 10mm, interactive stacking.
15, the working voltage: 220V ± 10% (AC), 50HZ
16, the work of the ambient temperature and humidity: 5-40 ℃ , 5% -95%
Fourth, the system overview
The main performance of the system equipment and technical indicators of the equipment and the application of the characteristics of the equipment
Amalgamation
System
Full-automatic fast panning system 1, one-button type full-automatic fast panning.
2. Amalgamation time is only 2 minutes, the whole experiment only takes 5 hours, no need to test period maintenance, suitable for emergency and laboratory operation.
The recovery rate of the equipment is up to 90%, which is the highest recovery rate of the post-detection equipment of the two insects in the world.
Immunomagnetic Separation System UniBest Company
Immunomagnetic Separation Kit for Two Worms
1. The procedure is simple, which greatly saves the manual operation time.
2, Even if the content of two insects in each concentrated water sample is low, the magnetic separation recovery can be maintained at more than 90%.
3. It can work well with the new generation of automatic washing equipment, which can greatly improve the washing efficiency and further improve the recovery rate of magnetic separation step.
1. It has passed the national standard GB5749-2006 and the U.S. EPA-1623 standard.
2. It can significantly improve the recovery rate of Cryptosporidium and Giardia.
3. It can reduce the background of residual debris during microscopic examination.
4. As low as 1 sporozoite/ oocyst can be detected.
UniBest Corporation
Sample Mixer
1. The mixing wheel can hold up to 12 tubes simultaneously.
2. Rotational speed 2-40rpm adjustable, digital display.
It is used to mix the magnetic beads and the sporocyst oocysts before the immunomagnetic separation step, so that they can be fully combined and the recovery rate of the two insects can be improved.
Magnetic separator (micro and large capacity) 1, large capacity magnetic separator working volume: 5-15 ml, with the side of the flat-wall test tube with special.
2, Micro magnetic separator working volume: 1.5ml; can be placed at the same time six standard 1.5ml microcentrifuge tube.
The magnetic separator is a special tool for the magnetic separation of two worm immunizations. When the test tube is inserted into the magnetic separator for magnetic particle concentration, the magnetic beads will be attracted to the test tube wall by the magnetic field. As a result, the upper layer of clear liquid and impurities can then be smoothly removed to separate it from the magnetic beads and the two worms.
Side Flat-Wall Tubes for Two WormsSide Flat-Wall Tubes with Large Capacity Magnetic Separator are specially designed for two-worm immunomagnetic separation experiments.
IFA Staining
System
UniBest IFA
(Indirect Immunofluorescence)? Staining kit
1, Fast and accurate detection.
2, the reagent is cost-effective, and the use of special solid color solution so that the specimen does not fade, the fluorescence effect can be maintained for several days in order to re-examination.
3, the reagent shelf life is usually more than 24 months.
UniBest Two Worms fluorescent reagent is a special immunoglobulin M pentamer, by five FITC binding sites at the same time labeled with an antibody, with significant bright fluorescence performance, and fluorescence effect of the industry to maintain a long time.
The QC sample comes standard with 5 doses for positive control experiments, making the results more accurate and reliable.
Microscopy
System
Fluorescence microscope 1, sampling two insects special fluorescence microscope.
2, equipped with high-end imaging equipment, so that the imaging effect is obvious.
This set of microscopy system can well complete the bright field and dark field microscopic observation, with good reliability, stability.