Does the EO Sterilization Indicator Card have to be affixed? Is there a regulatory or standard requirement?

The EO Chemistry Indicator Card only means that the product has been sterilized by EO, it does not mean that it is sterile. A 7-day test is required to determine if the product is sterilized.

There are no regulations or standards that mandate the EO chemical indicator card.

But using a chemical indicator card is the easiest and most intuitive way to distinguish whether a product is sterilized or not.