1,pcr laboratory area to choose the construction materials: the wall should choose a solid structure, airtight, no dead ends, the wall is easy to clean and easy to disinfect, no dust, corrosion-resistant and other characteristics. Ground: choose pvc coil or epoxy resin self-leveling corrosion-resistant, easy to clean materials.
2, ready to prepare amplification analysis, in order to avoid cross-contamination, the experimental area design full exhaust, full fresh air system requirements, to maintain a stepped differential pressure (with positive to negative pressure). Realize the process, goods, personnel, air pressure airflow one-way process protection.
3, pcr laboratory is not too strict cleanliness requirements, can be based on a variety of factors to consider.
Basic function introduction:
1, the preparation area, the preparation of reagents required for the experiment, such as subassembly, transported by the transfer window to the preparation area, rigorously take the personnel channel.
2, the preparation area, mainly for the preservation of samples, etc., the extraction of rna\dna, storage and its addition to the amplification reaction tube and determination of rna\dna synthesis.
3, amplification area, dna amplification, to prepare the dna template and synthesis of cDNA and the addition of the main plate reaction mixture prepared into a reaction mixture, etc. can also be carried out in the amplification area, in the nested pcr assay, usually in the first round of amplification after the reaction tube must be opened, so the nested amplification of the danger of higher contamination, the second time to add samples must be completed in the area.
4, amplification analysis, mainly used to do the determination of amplification products, this area is the main source of amplification product contamination, so it is necessary to avoid the idea of avoiding the amplification product through the articles or work clothes will be taken out of the area.