Optimization steps of liquid mass method

GC-MS is an analytical instrument combining gas chromatography with mass spectrometry, which consists of gas chromatograph and mass spectrometer through an interface. In GC-MS, the gas chromatograph is equivalent to the sample pretreatment machine of the mass spectrometer, and the mass spectrometer is the detector of the gas chromatograph, which are organically combined through the interface. Therefore, the interface is the key equipment of gas chromatography-mass spectrometry.

Liquid chromatography-mass spectrometry (HPLC-MS) is also called liquid chromatography-mass spectrometry. It uses liquid chromatography as a separation system and mass spectrometry as a detection system. The sample is separated from the mobile phase in the mass spectrometry part, and the ion fragments are separated by the mass analyzer of mass spectrometry after ionization, and the mass spectrometry is obtained by the detector.

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The advantage of chromatography is separation, which provides the most effective choice for the separation of mixtures. However, it is difficult to obtain the structural information of substances, and it mainly depends on the comparison with standard samples to judge unknown substances. The detection of compounds without ultraviolet absorption needs to be analyzed by other means. Mass spectrometry can provide structural information of substances, and the amount of samples used is very small, but the samples to be analyzed need to be purified and have a certain purity before they can be directly analyzed. Therefore, it is expected to combine chromatography and mass spectrometry to make up for their respective shortcomings.

Reference to the above content: Baidu Encyclopedia-LC-MS.