Autoclave steam sterilization conditions are: 121.3 ℃, 15 ~ 30min.
I. Principle:
Autoclave steam sterilization is one of the most commonly used, the most effective method of sterilization, usually the pressure at 103 .4kPa, the temperature inside the container 121 .3 ℃, time 15 ~ 30min, using the High temperature and high pressure sterilization, not only can kill general bacteria, fungi and other microorganisms, germ cells, spores also have a killing effect, is the most reliable, the most commonly used physical sterilization method.
Two, commonly used:
Mainly used for items that can withstand high temperatures, such as culture media, metal instruments, glass, enamel, dressings, rubber and sterilization of some drugs. High-pressure steam sterilizer types and styles more, the following exhaust pressure steam sterilizer is commonly used sterilization equipment, the pressure rises to 103.4kPa (1.05kg/cm2) temperature up to 121.3°C, maintained for 15 to 30 minutes, can achieve the purpose of sterilization.
Determination of sterilization effect:
1, thermocouple test method:
The use of thermocouples will be inserted into the package of items with a heat-sensitive electrodes, through the change in the current response to the action of the measured temperature, can be traced from the temperature recorder on the record paper to observe the temperature curve of the entire sterilization process. New high-pressure steam sterilizer with thermocouple and temperature recorder device.
2, stay thermometer test method:
Leave the maximum temperature of the thermometer indicates 160 ℃, the use of its mercury column first thrown to 50 ℃ below, placed in the sterilized items, sterilized only after the completion of the observation of the number of thermometers can be taken out, is the shortcomings of the thermometer.
3, chemical indicator test method:
Some of the melting point close to the temperature required for autoclaving chemical substance crystal powder into a small glass tube, closed the mouth of the tube on the fire, made into an indicator tube. Sterilization of the indicator tube into the items, sterilization is completed to take out the indicator tube, such as the chemical substances have melted, indicating that the temperature in the sterilization room to reach the temperature indicated by the indicator tube.
The melting point of commonly used chemicals are: benzoic acid phenol, 110 ℃; antipyrine, 111~113 ℃; acetanilide, 113~115 ℃; succinic anhydride, 118~120 ℃; benzoic acid, 121~123 ℃; sulfur powder melting point of 121 ℃, but most of the hospitals in the country used the sulfur melting point of 114~116 ℃, the lowest only 111.2 ℃. The lowest is only 111.2℃.