Differences:
1. Concepts and basic principles
Immunohistochemistry, also known as immunocytochemistry, is a new technique for qualitative, localization, and quantitative determination of the corresponding antigens through the antigen-antibody reaction and histochemical chromogenic reaction of the specific antibody labeled with a chromogenic agent in the histochemical cells in situ. It skillfully combines the specificity of immune reaction and the visibility of histochemistry, and with the help of microscope's visualization and magnification, it can detect various antigenic substances at cellular and subcellular levels, and display the corresponding genes and gene expression products in situ. Immunohistochemistry technology now has: immunofluorescence tissue (cell) chemistry, immunoenzymatic tissue (cell) chemistry, affinity histochemistry, immunogold and silver and iron labeling immunohistochemistry technology.
Immunofluorescence tissue (cell) chemistry technology is the use of fluorescein-labeled known antibody (or antigen) as a probe, the detection of tissue to be tested, cell specimens in the target antigen (or antibody), the formation of the antigen-antibody complex with fluorescein, under a fluorescence microscope, due to high-pressure mercury lamp light source of ultraviolet irradiation, fluorescein emits a bright fluorescence, so that you can distinguish the antigen (or antibody) location and its location. The location and nature of the antigen (or antibody) can be distinguished, and the content of the antigen can be calculated using the fluorescence quantification technique. In order to achieve the antigenic material positioning, qualitative and quantitative determination of the purpose.
2. Specimen production: immunofluorescence is generally used frozen section, to reduce the interference of impurities; and enzyme immunohistochemistry is generally used paraffin section or frozen section can be.
3. Experimental steps: Immunofluorescence staining is simple, while enzyme immunohistochemistry is more complex, more DAB color development process.
4. Preservation of stained specimens: immunofluorescence stained specimens are usually photographed for a short period of time, and the fluorescence declines after a long period of time, while enzyme immunohistochemistry stained specimens can be preserved for a long period of time.
5. In addition to knowing whether the proteins are expressed in the plasma or the cell membrane, immunohistochemistry results can also be used to do relative quantitative analysis.
6. Immunofluorescence pictures are colorful and beautiful.
Yiqiao Shenzhou has professional technicians and instrument platforms such as *** focusing microscope to provide customers with high-quality immunofluorescence detection services, including immunofluorescence single staining, double staining, and multiple staining on cell crawls, paraffin sections, frozen sections and tissue microarrays, etc.