PCR (polymerase chain reaction) laboratory is also known as the gene amplification laboratory
"Interim Measures for the Management of Clinical Gene Amplification Test Laboratory" (Wei Medical Development [2002] No. 10)
"Clinical Gene Amplification Test Laboratory Work Specification" (Wei Inspection [2002] No. 8)
Two, the basic requirements of nucleic acid laboratory design SICOLAB
1, reagent preparation area: PCR laboratory is the most "clean" area, it should not have any nucleic acid components, otherwise it will seriously affect the accuracy of the test results. Specimen preparation should be carried out in the biological safety cabinet.
Major equipment: refrigerator, cryogenic refrigerator, mixer, micro-sampler (0.2-1000ul), mobile ultraviolet lamp.
2, sample preparation area: nucleic acid extraction, storage, amplification reaction tube production.
Major equipment: refrigerator, ultra-low temperature refrigerator, high-speed freezing centrifuge, mixer, micro-sampler (0.2-1000ul), mobile ultraviolet lamp, water bath, biological safety cabinet.
3. Amplification zone: the configuration of amplification reaction system and the extraction and addition of nucleic acids should be carried out in the amplification zone.
Main equipment: amplifier, centrifuge, micro-sampler (0.2-1000ul), mobile ultraviolet lamp.
4. Amplification product analysis area: the last working area in the product analysis area is used for the analysis of amplified fragments.
Major equipment: gel imaging analyzer, electrophoresis instrument, electrophoresis tank, micro-sampler (0.2-1000ul), mobile UV lamp.