Special laboratory large-scale instrumentation laboratory should do what standards

Selection of the site of the second-level biosafety laboratory 1 on the site, although the second-level biosafety laboratory has no special requirements, but from the perspective of biosafety, the laboratory is located in a more ideal location. Main laboratory technical parameters: 1, cleanlinessClass8 greater than or equal to 0.5μm of dust particles greater than or equal to 352000 particles / m3 (352 / L) to less than or equal to 352000 particles / m3 (3520 particles / L), greater than or equal to 3μm dust particles greater than or equal to 3000 particles / m3 (3 / L) to less than or equal to 30000 particles / m3 (30 particles / L), greater than or equal to 3μm dust particles greater than or equal to 30000 particles / m3 (3 / L) to less than or equal to 30000 particles / m3 (30 particles / L). m3 (30 particles / L). 2, illumination: minimum ≥ 300Lx, 3, noise: ≤ 60dB4, relative humidity: 30% ~ 65%5, temperature: 18-25 ℃ 6, differential pressure: -10Pa7, airflow direction: single-phase flow, from the clean area to the contaminated area 8, walls, ceilings: color steel sheet: steel thickness of 0.426, 15 grams of film 9, aluminum alloy: electrophoresis material for windows and doors, the rest are sprayed. 10, aluminum alloy: electrophoresis material for windows and doors. The rest are sprayed. 10, purification door: with observation window, door closer 11, high-efficiency air supply outlet: stainless steel stamping 12, floor: PVC coil 13, wash basin: stainless steel, containing sensor faucet, automatic hand dryer 14, transfer window: stainless steel mechanical chain 15, eyewash: stainless steel desktop 16, blower group: containing fresh air outlet, fresh air return air mixing section, air supply section, even flow section (maintenance section), mid-flow section (maintenance section), and the contaminated area. Flow section (maintenance section), medium-effect section, air section, anechoic section 17, exhaust air unit: the exit has a high-efficiency filter filtration 18, operating table: veneer physical and chemical board 19, the entrance to the international general biological safety hazard symbols, while there is a plexiglass plate labeled with the name of the laboratory, the person in charge of the precautionary measures, the emergency contact information. At the exit there is a safety exit sign that is clearly recognizable in the dark. Enclosure BSL-1 Laboratory 1) No special siting is required, an ordinary building is sufficient, but it should be designed to prevent the entry of arthropods and rodents. 2) A hand-washing sink should be provided in each laboratory, and it is desirable to set it close to the exit. 3) Clothes-hanging devices should be provided at the entrance of the laboratory, and the personal civilian clothes should be placed separately from the laboratory uniforms. 4) The laboratory's walls, ceilings, and floor should be smooth, easy to clean, impermeable to water, resistant to chemicals and disinfectants. The floor should be non-slip and should not be carpeted.5) The laboratory countertop should be waterproof, corrosion-resistant, and heat-resistant.6) Kitchen cabinets and lab benches in the laboratory should be solid. Kitchen cabinets and lab benches should be kept at a distance from each other to facilitate cleaning.7)Laboratories with operable windows, if any, should be screened.8)Working lighting should be ensured in the laboratory to avoid unnecessary reflections and glare.9)There should be proper disinfection equipment.BSL-2 Laboratories1)Meet the requirements of BSL-1.2)Laboratory doors should be lockable and self-closing.3)Laboratory door should be lockable and self-closing.4)Laboratory doors should have a locking device. . The laboratory door should have a viewable window.3) There should be adequate storage space for items to be placed for ease of use. Storage space for long-term use should also be available outside the laboratory work area.4) Specialized work clothing should be used in the laboratory; latex gloves should be worn.5) There should be storage for personal clothing outside the laboratory work area.6) Autoclaves should be provided in the building where the laboratory is located and should be inspected and verified on a regular basis to ensure compliance.7) Biosafety cabinets should be provided in the laboratory. Eyewash facilities should be provided, and emergency sprinklers should be available if necessary.9) It should be ventilated, and if windows are used for natural ventilation, there should be insect screens.10) There should be a reliable supply of electricity and emergency lighting. If necessary, important equipment such as incubators, biological safety cabinets, refrigerators, etc. should be equipped with power supply. 11) Laboratory exits should be in the dark can be clearly recognized signs. The following categories are described in detail as follows:1 The laboratory enclosure is made of color steel plate material, with smooth surface, corrosion resistance, waterproof, and all the seams are reliably sealed for easy cleaning and disinfection, and shockproof and fireproof.2 The intersecting corners between the ceiling, floor, and walls are rounded and reliably sealed.3 The floor is made of PVC material, leak-proof, no seams, polished, and non-slip.4 All the doors of the laboratory can be closed automatically.5 The laboratory bench top is made of physical and chemical board, and all the doors can be closed automatically.6 The laboratory bench top is made of physical and chemical board, and all the doors can be closed automatically. The laboratory bench tops are made of physicochemical boards, which are waterproof, corrosion-resistant, and heat-resistant.6 All entrances are protected from arthropods and rodents.7 Laboratory exits are marked with signs that are clearly recognizable in the dark. Plane structure and partitioning1Partitioning: divided into clean, semi-contaminated and contaminated areas.1.1 Clean area: used for public activities and rest areas for laboratory personnel.1.2 Semi-contaminated area: i.e., buffer corridor used for specimen transfer, waste output, and access to the locker room.1.3 Contaminated area: the core area of the laboratory used for experimental operations.2 Setting up: including distribution boxes, air conditioners, lockers, class II biological safety cabinets, laboratory tables, hand-washing sinks, eye washers, transfer windows, etc. Laboratory Sterilization and Isolation Requirements1 Requirements 1.1 The laboratory is relatively independent and separated from the public **** parts by isolation doors. The experimental core area should include the laboratory room and with the connected buffer corridor, clearly divided into clean, semi-contaminated and contaminated areas.1.2 All items in the laboratory should be dedicated to the need to take out must be strictly disinfected.1.3 Laboratory staff entering the laboratory should be wearing a good personal protective equipment.1.4 culture media, tissues, body fluids, etc., must be placed in leak-proof airtight containers for storage and transportation.1.5 serological tests, Pathogenic bacteria testing, sample processing and dispensing should be carried out in Class II biological safety cabinet.1.6 When centrifuging samples, the centrifuge should be placed in a detoxification cabinet or biological safety cabinet to prevent the generation of aerosols or spattering.1.7 The equipment used in the course of experiments and experimental wastes should be sterilized and disinfected in accordance with the provisions of the regulations.1.8 After completion of the experiments, disinfect the surface of the objects, then take off the personal protective equipment and carry out air disinfection in accordance with the prescribed procedures.1.9 The laboratory staff should wear personal protective equipment when entering the laboratory. 1.9 Before leaving the laboratory, hands must be cleaned and disinfected according to relevant regulations. 1.10 Instruments and equipment must be strictly disinfected according to relevant regulations before transporting them out of the laboratory.2 Disinfection Methods2.1 Disinfection of indoor air in the laboratory2.1.1 Air supply and exhaust systems can be used to enhance ventilation.2.1.2 Ultraviolet lamps, lamp carts are suitable for unmanned indoor air, object surface disinfection. Commonly used indoor suspended ultraviolet lamps for indoor air disinfection when the number of installations for an average of 1.5W/m3 (irradiation intensity ≥ 70MW/cm2), irradiation time of not less than 60 minutes.2.2 Laboratory Surface Disinfection 2.2.1 Ground Laboratory floors should be wet mopping and sweeping, dry mopping and dry sweeping is prohibited. When disinfection is required, spray or mop the floor with chlorine disinfectant with effective chlorine of 500-1000mg/L, and the dosage of disinfectant is not less than 100ml/m3. The mop should be dedicated to the polluted area and the clean area, and should not be mixed. After use, soak in the above disinfectant solution for 30 minutes, then clean with water, hang to dry, preferably in the sun after drying for use.2.2.2 Disinfection of object surfaces laboratory tables, tables, chairs, stools, door knobs, etc. can be used to effective chlorine for 500-1000mg / l chlorine-containing disinfectant spraying or wiping, and the role of the 10-15 minutes after the timely use of water to scrub, in order to remove residual disinfectant.2.2.2 .3 hand disinfection laboratory work should be immediately after the completion of hand washing and disinfection. Adopt sensor-type automatic hand-washing equipment, use bottled liquid soap, after washing hands can be used once paper towel wiping, preferably with a dryer. Before leaving the laboratory, after taking off the overalls, the hands should be cleaned.2.2.4 Pathogenic microorganisms experimental sewage disinfection in accordance with GB(8466-2001 "medical institutions sewage discharge requirements". Cleaning management system 1 principles of laboratory cleaning laboratory cleaning should be carried out under the premise of ensuring biological safety, in line with the requirements of biosafety protection, to comply with the principle of disinfection before cleaning. In general, biosafety laboratories should be disinfected by specialized personnel, but each laboratory staff member has the responsibility to do a good job of cleaning and disinfecting the laboratory.2 Cleaning and Disinfection SuppliesThe laboratory should be equipped with necessary cleaning and disinfection supplies, which mainly include mops, rags or gauze, buckets, disinfectant, prepared 75% ethanol, and so on.3 Disinfectants are mainly peracetic acid, chlorine disinfectant, etc.4 Disinfecting Apparatus Mainly UV lamps and UV lamp carts. The penetrating ability of ultraviolet light is particularly weak, so UV lamps must be cleaned regularly at regular intervals. Generally speaking, use a rag soaked with 75% ethanol to wipe the lamp once a month, and before wiping, unplug it and cool it for 10 minutes; the irradiation intensity of the ultraviolet lamp should also be tested frequently, and when the irradiation intensity is lower than 70% of the original irradiation intensity, the lamp should be replaced by a professional, and the irradiation intensity of the ultraviolet lamp should be inspected once every three months in general.5 Maintaining the tidiness of the laboratory environmentThe lab is prohibited from Eating, smoking, meeting guests and clamor. After each job is completed, the countertops must be cleaned and the laboratory restored to a state of pending operation. Laboratory countertops and instruments should be promptly wiped down with a disinfectant solution to wipe down the surface after each experimental operation is completed.6 Cleaning the Laboratory FloorThe laboratory floor should be wet mopped and swept, and dry mopping and dry sweeping are prohibited. Need to disinfect, available 0.1% peroxyacetic acid or effective chlorine 500-1000mg / l of chlorine disinfectant spray or mopping, disinfectant dosage shall not be less than 100ml / m2. contaminated area and clean area mop should be dedicated, not mixed. After use, soak in the above disinfectant solution for 30 minutes, then clean with water, hang to dry, preferably in the sun for use. Mop the floor with disinfectant solution by the staff after each experiment, and clean it thoroughly once a month.7 Laboratory tables, desks, chairs and stools. Door handles can be cleaned with 0.1% peroxyacetic acid mopping or effective chlorine 500-1000mg/l chlorine disinfectant solution spray or wipe, the role of 10-15 minutes after the timely scrubbing with water to remove residual disinfectant.8 textile cleaning cotton woven overalls, hats, masks, etc. into a special bag of dirt, with more than 70 ℃ hot water plus detergent washing; when there is obvious contamination of pathogenic microorganisms If there is obvious pathogenic microbial contamination, spray disinfectant solution at any time to disinfect or put into a special bag of dirt, autoclave sterilization treatment.9 Instrumentation and equipment can be cleaned with disinfectant solution or 75% ethanol wipe the surface disinfection, and then scrubbed with neutral detergent and water to remove the stains.10 hand washing laboratory work should be immediately after the completion of the cleaning and disinfection of the hands. Use sensor-type automatic hand-washing equipment, use bottled liquid soap, and wash your hands once with a paper towel, preferably with a dryer. Before leaving the laboratory, hands should be washed after removing coveralls. Disinfection and isolation system1 Timely disinfection1.1 When experimental microorganisms contaminate the experimental environment, the experiment should be stopped immediately and disinfected with an effective disinfectant solution.1.2 When the laboratory is contaminated by airborne and aerosol-borne pathogenic microorganisms, the laboratory should be shut down immediately for disinfection.1.3 Suspected contaminated items should be thoroughly sterilized or disinfected before they are taken out of the biosafety laboratory. Strains and related samples should be strictly sterilized or disinfected on the surface of the carriers and packaging containers of the samples before they are taken out, and the carriers should be sterilized again when the samples are taken out, and the packaging containers should be sterilized or disinfected.1.4 Containers holding contaminated microorganisms experimental consumables should have sufficient amount of disinfectant, and used consumables should be immediately immersed into disinfectant.1.5 Immediately after the experiment is finished, the experimental The environment should be disinfected immediately after the completion of the experiment, such as scrubbing, disinfection of the work surface, the ground, turn on the ultraviolet lamp irradiation for more than 1 hour.1.6 After the completion of the experiment, the experimental waste should be immediately autoclaved or disinfected and other harmless treatment.1.7 Experimental personnel should be cleaned and disinfected immediately after the end of the experiment, wash their hands and face, and take a bath and change their clothes if necessary.2 Thoroughly disinfected 2.1 The items to be disinfected should be taken to disinfect thoroughly. measures, leaving no dead ends.2.2 Disinfectant immersion disinfection, the items to be disinfected all immersed in disinfectant, for items with a lumen, the disinfectant should be poured into the lumen to exclude the air embolus.2.3 Disinfectant wipe disinfection, disinfectant wipe all surfaces of the items needing to be disinfected and keep the full effect of the time.2.4 Disinfection of the object to be selected according to the test, where possible contact with the test microorganisms The experimental equipment, test environment (including table, floor, walls, air), personnel (individuals and protective equipment, etc.) should be taken accordingly disinfection.3 Effective disinfection 3.1 According to the disinfection object and microbial species to choose the appropriate method of disinfection and sterilization.3.1.1 For bacterial spores, fungal spores contamination of the items, the choice of high-level disinfection or sterilization method. For equipment contaminated by high resistance pathogenic factors must be disinfected with sodium hydroxide and high-pressure steam (136°C, 60 minutes). 3.1.2 For items contaminated by pathogenic microorganisms such as mycobacteria, fungi, hydrophilic viruses, spirochetes, mycoplasma, chlamydia, etc., select a medium-level or higher level of disinfection method. 3.1.3 For items contaminated by general bacterial propagules, lipophilic viruses, etc. 3.1.3 For articles contaminated by general bacterial propagules, lipophilic viruses, etc., choose medium-level or low-level disinfection methods. 3.14 When disinfecting articles with more organic matter (e.g., blood, secretions, excretions), the dosage of disinfectant drugs and/or the duration of the action should be increased. 3.1.5 When microbial contamination of the disinfected articles is particularly serious, the dosage of disinfectant drugs and/or the duration of the action should be increased. 3.1.6 When choosing surface disinfection methods, the nature of the surface should be taken into consideration. 3.1.6 When choosing surface disinfection methods, the nature of the surface should be taken into consideration. Smooth surfaces can be irradiated by ultraviolet ray disinfector or wiped with disinfectant solution, and porous surfaces can be disinfected by spraying. 3.2 Disinfecting instruments and disinfectant solution should be approved by health permit. 3.2.1 Disinfecting products should be used within the validity period, and disinfection should be carried out in accordance with the instructions for the indicated action concentration (or intensity), time of action and method of use. Continuous use of disinfectants should be regularly tested for active ingredient content and timely replacement. 3.2.2 Disinfection, sterilization equipment should be regularly measured disinfection or sterilization effect. Large-scale disinfecting and sterilizing equipment should pass performance tests before formal use and after overhaul to prove that it can be used effectively.4 Precautions4.1 It is necessary to reduce the harm of disinfecting treatments to human beings, the destruction of articles, and the impact on the environment.4.2 According to the physicochemical properties of the articles to be disinfected, select a disinfectant with good compatibility. High-temperature, moisture-resistant articles and equipment, preferred autoclave sterilization; metal, glass equipment, oils and dry powders can be selected dry heat sterilization. Fear of heat, fear of moisture, fear of corrosion and valuables, can choose ethylene oxide fumigation disinfection, sterilization. 4.3 disinfectant for human disinfection should be selected for human skin and mucous membrane stimulation less disinfectant. 4.4 disinfectant for environmental disinfection, disinfectant should be strictly in accordance with the disinfection of the object, the type of target pollutants and the degree of contamination in order to achieve effective disinfection, and does not contaminate the environment as a prerequisite for the reasonable choice of disinfectant concentration and the time of action. Rational choice of disinfectant concentration and time of action. Laboratory waste management 1 after the experiment waste if not disposed of in a timely manner will pollute the environment, thus causing harm to the staff, so the relevant personnel must be strictly in accordance with the provisions of the implementation of the 2 experimental samples in the processing and laboratory testing of waste, such as flatware, pipette boxes, plastic test tubes, etc. should be placed in appropriate containers or strict leak-proof high-pressure bag. 3 experimental process and birth of contaminated liquid materials, discarded liquid specimens, culture culture tubes, and so on. The liquid specimens, cultures, etc. produced and generated during the experiments should be placed in special containers with disinfectant solution that is strictly leak-proof and covered in a timely manner.4 Sharp instruments that must be used to carry out the experiments, such as disposable syringes, needles, micropipette tips, glass instruments, surgical blades, and broken glass must be put into designated special containers with solid walls and sealed with lids.5 All the containers above that have wastes in them should be sent to autoclave after the completion of each experiment.6 The laboratory should also send autoclave for sterilization. All laboratory garbage and used latex gloves, isolation gowns, masks, disposable caps, etc., should be put in the corresponding dirt bags and discarded after autoclaving.7 All clinical specimens should be autoclaved after the retention period for subsequent processing.8 The autoclave should be under the responsibility of a special person, and operated in accordance with the instructions to ensure that it is at least 30 minutes of high-pressure at 121℃ to meet the requirements for sterilization and disinfection. The autoclave should be operated in strict accordance with the instructions for use to ensure that the autoclave is operated at 121℃ for at least 30 minutes in order to meet the requirements for sterilization and disinfection.9 All the wastes generated from the experiments must be strictly sterilized by autoclaving before they can be transported out of the experimental area and sent to the designated place for centralized incineration.10 Empty chemical containers 10.1 Non-hazardous empty chemical containers: sterilized in accordance with the general rules and then classified and disposed of according to the general wastes. 10.2 Hazardous empty chemicals: Hazardous empty chemical containers shall not be disposed of as ordinary waste. Dispose of them in a harmless manner accordingly to the relevant hazardous chemicals disposal law or by the higher authority.11 If you encounter any situation not mentioned in this operation guideline, please contact the laboratory safety department. Routine Safety Operating Procedures1 Non-staff members are prohibited from entering the laboratory, and special circumstances such as visiting the laboratory must be approved by the laboratory director.2 Eating, drinking, smoking, handling contact lenses, applying make-up, and storing food and daily necessities are strictly prohibited in the laboratory workspace.3 Items unrelated to experiments are not allowed to be brought into the laboratory.4 Wash your hands after contacting microorganisms or items containing microorganisms; take off before leaving the laboratory and wash your hands.5 During the experiments, please contact the laboratory safety department for more information. and wash hands.5 During experiments, operate in strict accordance with relevant operating procedures to reduce spills and aerosol production.6 Disinfect work surfaces at least once a day, and disinfect active substances at any time after spills.7 Suck up liquids with pipettes, and mouth suction is strictly prohibited.8 All cultures and wastes must be feasibly disinfected or inactivated, e.g., autoclave sterilized, before they are shipped out of the laboratory. Items to be transported out of the laboratory for disinfection should be placed in leak-proof special closed containers.9 Develop safe operating procedures for sharp instruments.10 Develop effective rodent and insect control measures.11 Laboratory entrances shall be labeled with eye-catching biohazard signs indicating the hazard factor, the biosafety level, and the name and telephone number of the person in charge of the laboratory and other relevant persons in charge. Chapter II special safety operation procedures.1 The laboratory is relatively independent, clearly divided into clean, semi-contaminated and contaminated areas, and the three areas shall not be intersected, and the flow of people and logistics shall be separated.2 When conducting infectious experiments, others are prohibited from entering the laboratory, or must be agreed by the director of the laboratory to enter; immune-tolerant or immunosuppressant staff members are not allowed to enter the laboratory, or must be agreed by the director of the laboratory to enter. Staff should be immunized and tested as necessary (e.g., Hepatitis B vaccine, BCG, etc.).4 Basic serum retention should be collected from staff engaged in hazardous work when necessary, and serum samples should be collected regularly as needed, tested, and a report issued, with any problems resolved in a timely manner.5 Laboratory director should develop rules and regulations and biosafety procedures to be included in the biosafety manual, which will be kept in the special custody of the laboratory manager. Staff should read it before entering the laboratory and follow it in their work; other personnel should be informed of the potential risks and meet the requirements for entering the laboratory, and be allowed to enter only with the permission of the Laboratory Director.6 Staff should be trained in the knowledge of the potential hazards involved, and in the procedures for preventing exposures and disinfecting after exposures; and they should be given up-to-date training once a year.7 The use of sharps in the laboratory is prohibited, except for special circumstances. Whenever possible, plastic equipment should be used instead of glass equipment, and when it is necessary to use them, the safe operation procedures for sharps should be strictly adhered to.8 Culture media, tissues, body fluids, and other potentially hazardous wastes shall be disposed of in accordance with the waste disposal system.9 Personnel who are exposed to infectious substances shall report to the laboratory manager in a timely manner and record the accident and the treatment plan.10 It is prohibited to bring extraneous animals into the laboratory.11 Experiments shall be performed using accepted and qualified equipment during the experimental process. process should use the acceptance of qualified secondary biological safety cabinets.12 Experimental equipment must be strictly sterilized before shipping out for repair or maintenance. As for approval, it must be approved. But not necessarily the government. If it is a university laboratory, to be approved by the Ministry of Education. If it's a provincial government department, then it has to be approved by the State Council. It's not the same. As for the goose's vaccine, it's impossible to know. That's a state secret, and it would be a crime to tell you.