Methods for making your own nucleic acids at home are described as follows:
First, buy a self-test nucleic acid test kit.
1, clean hands: before antigen detection, hands should be strictly sterilized and cleaned. Then check whether the package of the kit is intact and broken.
2. Sample collection: Clean the nasal cavity and tilt the head back slightly. The end of the hand-held nasal swab entered from one nostril, slowly penetrated deeper into the nasal cavity, rotated inside the nasal cavity at least 4 times, and then repeated this operation in the other side of the nasal cavity.
3. Sample extraction: Immediately after sampling, the nasal swab was placed into the sampling tube, and the swab was rotated vigorously to fully mix with the treatment solution.
4. Detection: break the cap of the treatment bottle, take out the test card, invert the treatment bottle vertically, put three drops of liquid into the sample hole and wait for fifteen minutes. Detection of the card at the C shows color and T does not show for negative, if the C and T show color for positive, T scale line a bar on behalf of the test is invalid.
5, waste disposal: to test items into a medical sealed bag, according to medical waste disposal.
Antigen detection principle:
The corresponding viral protein antigen needs to be fixed in colloidal gold or latex and other carriers, if the sample contains viral proteins, these proteins will be combined with the antibodies on the carrier, thus making the test paper change color. The novel coronavirus antigen test is typically used during the acute infection phase, and a positive result can be used for early transfer and rapid management of suspected populations, but it cannot be used as a basis for diagnosing novel coronavirus infection.