Commonly used hygrothermal sterilization methods are steam sterilization, high-temperature hygrothermal sterilization, boiling sterilization and so on.
1, steam sterilization (hygrothermal sterilization)
The use of high temperature and high humidity steam sterilization of items. Commonly used steam sterilization equipment, including pressure steam sterilizer (commonly used for high-pressure sterilizer), air circulation sterilizer. Steam sterilization is suitable for heat-resistant, moisture-resistant instruments, containers and other items sterilized.
2, boiling sterilization
The items will be placed in boiling water for sterilization. Boiling water can achieve the effect of sterilization, but for some instruments and articles that require high heat resistance, boiling sterilization may not be thorough enough.
3, high-temperature moist heat sterilization
The items will be put into the heating of high-temperature water for sterilization. By heating the water temperature and time to achieve the sterilization effect. High-temperature moist heat sterilization is suitable for some of the items adapted to water, such as syringes, needles and so on.
Historical overview of the discovery of hygrothermal sterilization:
1. In the late 18th century to the early 19th century, the French chemist Louis Pasteur and the British doctor Joseph Lister pioneered the concept of aseptic laboratories and aseptic surgery, laying the foundation for the discovery of hygrothermal sterilization.
2. At the end of the 19th century and the beginning of the 20th century, the German microbiologist Robert Koch discovered that high temperature and high humidity could effectively kill microorganisms by studying the resistance and death patterns of bacteria.
3. In the early to mid-20th century, German microbiologist Richard Petry and American physician Arnald Renick explored the specific parameters and application methods of moist heat sterilization under laboratory conditions.
4. In the 1940s, American microbiologist Charles Sheridan discovered that moist heat sterilization also has an inactivating effect on viruses, providing a technical basis for the development of virus vaccines.
5, with the continuous progress of science and technology and the improvement of experimental conditions, hygrothermal sterilization method in the medical and laboratory is widely used, and gradually formed a standardized procedure and operation norms.
Reference: Baidu Encyclopedia - Moist Heat Sterilization Method