When detecting RNA, how to deal with the equipment needed for electrophoresis

Specifically, it is very complicated, you can refer to the book of molecular cloning, said very detailed! Next, I will introduce our Chifeng College-Medical School of Molecular Biology Laboratory method: 1. Generally, the plastic utensils, including pipette tips, with 0.1% DEPC said to be soaked for more than 12 hours (pipette tips with 0.1% DEPC at 37 ℃ under the condition of 120 rpm shaker shaking, the purpose of the pipette tip internal also be treated). Then wet heat autoclave sterilization, DEPC decomposition into carbon dioxide and water, drying can be. 2. electrophoresis tank we so deal with, with 10% NaOH, soak for 1 hour, rinse with enzyme-free water, can be (pay attention to the requirements of the environment, the fewer the better, the saliva and hair RNA enzyme prevention is not prudent to prevent)! 3. electrophoresis solution preparation, in the special operating table, the preparation of buffer with enzyme-free water (of course, is 0.5) Glassware, including triangular flasks for gel production, needless to say, closed and covered with tin foil, baked at 180℃ for more than 2 hours (5-6 hours if it is the first time)! Wish you success!