In the order of your question
First, the specification of the test tube is about the length of - cm, diameter of 2 - 2.5 cm, the length of the small glass tube is about 2-3 cm diameter of about 0.3-0.5 cm. operation is to put the small glass tube (one end is closed) open end of the large test tube towards the bottom. Put the open end of the small glass tube (closed at one end) towards the bottom of the large test tube, as to how many pieces are needed. Depends on how many samples you do at a time, a few times, we usually prepare 30 - 50 sets.
Second, no matter what the medium, we are our own preparation, reagent stores can be bought (according to the method listed in the reagent varieties to purchase.
Third, definitely need to add the plug, we use a soft material (like soft rubber?). White, another plug in the center of the plug can be active plug, can ensure that the test tube after plugging breathable, in fact, with what material is not important.
Fourth, the need for instrumentation: biochemical incubator, aseptic operating table (air essence level 100), autoclave, refrigerator, dry box.