What is a PCR instrument

PCR (polymerase chain reaction) is the use of DNA in vitro at 95 ° Celsius high temperature denaturation will become a single-stranded, low-temperature (often 60 ° C or so) when the primer and the single-stranded in accordance with the principle of base complementary pairing, and then adjust the temperature to the optimal reaction temperature of the DNA polymerase (72 ° C or so), DNA polymerase along the phosphoric acid to the pentose carbon sugar (5'-3') direction of the synthesis of complementary strands. The PCR instrument based on polymerase manufacturing is actually a temperature control device, which can be well controlled between denaturation temperature, reversion temperature, and extension temperature.