COVID-19's nucleic acid detection mainly uses RT-PCR detection kit operation is:
1. Detectives first use nucleic acid extraction kit to extract nucleic acid from patient samples.
2. Put the extracted nucleic acid into the detection reagent for replication.
3. Determination of results. Steps 1 and 2 have strict operating procedures and conditions, and the overall time consumption is between 1-4 hours, and it is finally decided.
Hospital tests are unified on the computer in the morning, and it takes about five hours to get a unified result, that is, in the afternoon, and then report it in a unified way, without on-site inspection. So the samples sent in the afternoon or evening were made the next day. Some hospitals don't have the laboratory conditions themselves and can't do it. They need to be sent to the next level laboratory. The conservative estimation time is 1-2 days.
Extended data
Principle of nucleic acid detection
All living things except prions contain nucleic acids, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Novel coronavirus is a virus containing only RNA, and the specific RNA sequence in the virus is a sign to distinguish the virus from other pathogens.
After the appearance of novel coronavirus, Chinese scientists completed the analysis of the whole genome sequence of novel coronavirus in a very short time, and found the specific nucleic acid sequence in novel coronavirus by comparing with the genome sequences of other species.
If novel coronavirus's specific nucleic acid sequence can be detected in patients' samples during clinical laboratory testing, it should be suggested that patients may be infected by novel coronavirus.
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