Laboratory biosafety management system
1.Laboratory requirements:
1.1 Specialized laboratories, the door has a clear sign of biological experimental safety, adequate operating space, the laboratory should be divided into clean and contaminated areas.
1.2 Laboratory countertop materials should be acid and alkali resistant, easy to clean and disinfect, no leakage of liquid.
1.3 The laboratory has a mobile ultraviolet lamp for air sterilization.
1.4 Disinfectants, disinfecting equipment and equipment are available.
1.5 There is a running water device for sensing, eyewash, eye masks, sufficient disposable gloves and masks are available.
1.6 Cleaning areas (rooms) are equipped with facilities for storing personal clothing and supplies.
1.7 Vent-free autoclave steam sterilizers are purchased for sterilization of medical waste.
1.8 The laboratory is equipped with air-conditioning equipment and the room temperature is maintained at 20~25℃.
2. Safe operation:
2.1 Personnel should be familiar with the knowledge of biosafety operation and sterilization techniques.
2.2 No food and drink, smoking and make-up dressing, meeting guests and other behaviors unrelated to experimental work are allowed in the laboratory.
2.3 The relevant supplies in the laboratory (including overalls) shall not be used for other purposes; private and unrelated items shall not be brought into the laboratory;
2.4 Gloves and overalls shall be worn when working, and gloves shall be discarded if they are worn out, washed hands, and replaced with new gloves.
2.5 Do not touch exposed skin, lips, eyes, ears, hair, etc. with gloved hands.
2.6 Avoid the use of sharp objects and utensils as much as possible; it is advisable to use supplies of unbreakable materials; it is forbidden to suck any substance by mouth.
2.7 After the work is finished, the worktable should be disinfected; when there are specimens and test reagents splashed during the operation, they should be disinfected in time; and the environment should be kept neat and clean in normal times.
2.8 After finishing work, take off gloves and wash hands, then take off work clothes and wash hands with liquid soap and running water.
2.9 When encountering accidents, they should be handled immediately in accordance with the procedures of the emergency plan for accidents.
3. Disinfection of Waste Items
3.1 Blood specimens and disposable bacterial culture media shall be disinfected with an autoclave sterilizer and then put into a sharps box, and disposed of as medical waste.
3.2 Sputum and fecal specimens are treated as medical waste.
3.3 Sharps such as needles, rotten slides, pipettes, etc., are placed in sharps boxes.
3.4 Recovered blood transfusion bags are disposed of as medical waste.
3.5 Disposable consumables that do not need to be recycled, such as pipette tips, straws, glue plugs, etc., are put directly into yellow medical waste bags without soaking in sterilized water.
3.6 All laboratory waste, should be strictly in accordance with the medical waste of domestic waste, domestic waste is placed separately.
4. Health monitoring
4.1 Laboratory staff must be tested for HIV antibodies and hepatitis B, hepatitis C and other hepatitis viral markers before engaging in work.
4.2 In case of occupational exposure accidents, the exposed person should take blood for HIV antibody test once at the time of exposure, and once in the 4th week, 8th week, 12th week and 6 months after exposure.
4.3 Those suffering from skin disorders, skin ulcers, and broken skin should be diagnosed and treated promptly. Accident Response and Emergency Procedures
Every laboratory working with infectious microorganisms should have safety precautions in place to address the hazards of the microorganisms and animals they work with.
A written program for dealing with accidents in laboratory and animal facilities is required in any laboratory involved in the handling or storage of microorganisms of Hazard Levels 3 and 4 (Biosafety Level 3 containment laboratories and Biosafety Level 4 maximum containment laboratories). The state and/or local health department is to be involved in the development of the emergency plan.
Accident Response Plan
The accident response plan should provide the following operational specifications:
1. Preparedness for natural disasters such as fires, floods, earthquakes, and explosions
2. Risk assessment of biohazards
3. Treatment of accidental exposures and decontamination
4. Emergency evacuation of people and animals from the site Evacuation
5, Emergency medical treatment of personnel exposure and injuries
6, Medical monitoring of exposed personnel
7, Clinical management of exposed personnel
8, Epidemiological investigations
9, Continued operations after an accident.
The following aspects should be considered when developing an accident response plan:
1. Identification of microorganisms with high hazard ratings
2. Locations of high-risk areas, such as laboratories, storerooms, and animal rooms
3. Specification of individuals and populations at risk
4. Specification of responsible personnel and their responsibilities, such as biosafety officers, safety personnel, local health departments, clinicians, microbiologists, veterinarians, epidemiologists, and fire and police departments
5. List units that can accept exposed or infected persons for treatment and isolation
6. Transfer of exposed or infected persons
7. List sources of immune serums, vaccines, medications, special instruments and supplies
8, supply of emergency equipment, such as protective clothing, disinfectants, chemical and biological spill kits, and equipment items for decontamination.
Anhui purification project in the people and purification is excellent. You can go on and take a look.