1. The appearance of the sterilizer and the sterilization chamber should be kept clean and dry.
2. Probe, water level meter should be cleaned regularly.
3. Door frame, rubber ring is not damaged, the steam inlet must not be blocked. It is best to apply talcum powder on the rubber strip after use every day. To extend the life of the rubber strip.
4. Door interlocking device should be flexible and reliable, open freely.
5. The trap is cleaned once a month to facilitate the discharge of cold air to maintain the temperature.
Two, overhaul cycle:
After one year of use, every year, we should ask the qualified testing department to do a comprehensive systematic inspection. Including: cylinder, door, piping system, electrical system. Safety valves, temperature gauges, pressure gauges should be calibrated regularly to ensure the safety and normal use of equipment.
Three. Operation steps :
1. First, take out the inner sterilization barrel, and then add the appropriate amount of water to the outer pot, so that the water level with the triangular shelf is appropriate.
2. Put back the sterilizing bucket and load the items to be sterilized. Be careful not to load too crowded, so as not to prevent the steam flow and affect the sterilization effect. Triangle flask and test tube mouth end are not in contact with the barrel wall, so as to avoid condensation wet the mouth of the paper and penetrate the cotton plug.
3. Add the lid and insert the exhaust hose on the lid into the exhaust slot of the inner sterilization barrel. And then two two symmetrical way at the same time tighten the two bolts opposite each other, so that the bolt loosening and tightening of the same, do not make the air leakage.
4. Use the electric stove or gas heating, and at the same time open the exhaust valve, so that the water boiling to exclude the cold air inside the pot. When the cold air is completely exhausted, close the exhaust valve, so that the temperature inside the pot with the increase in steam pressure and gradually rise. When the pressure in the pot rises to the desired pressure, control the heat source and maintain the pressure for the desired time. For this experiment, use 1.05kg/cm2, 121.3°C, 20 minutes to sterilize.
5. Sterilization of the required time, cut off the power supply or turn off the gas, so that the temperature inside the sterilizer naturally decline, when the pressure gauge pressure drops to 0, open the exhaust valve, loosen the bolt, open the lid, remove the sterilized items. If the pressure is not reduced to 0, open the exhaust valve, it will be due to the sudden drop in pressure in the pot, so that the medium in the container due to the internal and external pressure imbalance and rushed out of the mouth of the flask or test tube, resulting in the cotton plug stained with the medium and contamination occurs.
6. Will take out the sterilized medium into the 37 ℃ incubator culture for 24 hours, after checking if there is no growth of stray bacteria, can be used.
6.