Autoclave steam sterilization to achieve the temperature and maintenance time is generally between 15-30 minutes.
Autoclave steam sterilization is an effective sterilization method widely used in the fields of medical and biological products. In autoclaving, temperature and time are two important parameters that affect the sterilization effect.
Temperature is the absolute temperature reached during autoclaving.
In autoclaving, the temperature usually used is 121°C. This is because at this temperature, most microorganisms such as bacteria, fungi and viruses can be killed. Also, at this temperature, proteins are destroyed so that microorganisms cannot be revived.
Maintenance time is the time required to maintain the autoclave sterilization after reaching the sterilization temperature.
Depending on the sterilization needs, the maintenance time is generally between 15-30 minutes. The exact maintenance time will be affected by factors such as the type and quantity of the sterilized target material, the sealing and the specifications of the autoclave sterilization equipment.
In general, the sterilization effect of autoclaving is closely related to temperature and time.
If the temperature is too low or insufficient time, the sterilization effect may not be achieved; and if the temperature is too high or the time is too long, it may cause unnecessary damage to the sterilized material. Therefore, when autoclaving, the temperature and time need to be scientifically and reasonably controlled to ensure the reliability of the sterilization effect and the integrity of the sterilized material.
High-pressure sterilizer has two categories: one is the more advanced program-controlled pre-vacuum pressure steam sterilizer, developed countries abroad have been used. The sterilizer is equipped with an air extractor, used to pass into the steam before the first vacuum, to facilitate steam penetration. It has the advantages of short sterilization time and slight damage to the goods.
In the items placed in the crowded and overlapping situation can still be achieved sterilization, and even covered containers can be sterilized. The whole sterilization process adopts program control, which saves manpower and is stable and reliable. Domestic recently put into production JWZK-I2A program-controlled pre-vacuum pressure steam sterilization cabinet, good performance. Sterilization of the lowest vacuum for 8.0kPa (60mmHg), the highest temperature of 132 ~ 136 ℃.