Precautions
Precautions for calling the Citizen Service Hotline:
1. Callers must be clear about the facts of their claims and leave their real names and valid contact information;
2. If their claims are related to the scope of the Party Committee, the National People's Congress (NPC), the Political Consultative Conference (CPPCC), the military, or the judiciary, the citizens should reflect them directly to the petition and complaint departments of these units;
3. If the matter has entered the judicial process (the court has two trials or has been judged), the citizens should reflect directly to the Standing Committee of the Municipal People's Congress, the Political and Legal Committee of the Municipal Party Committee or the judicial organs of the higher level; for emergency help involving life and property safety, the citizens should directly dial 110, 119 and other emergency phones;
4. Parents should strengthen education and management of minors to prevent misdialing and misplacing; for invalid calls, the The agent will patiently persuade and counsel;
5. For malicious harassment phone calls, the public security department, once verified, will press the "? Public Security Administration Punishment Law" relevant provisions to pursue responsibility ?
The main acceptance
First, the *** departments and staff duties, procedures, administrative approval, administrative acceptance, investment promotion and other policies and regulations of the consultation;
Second, the city's reform and opening up, economic construction, urban construction and management of the views and suggestions;
Third, the social life of the occurrence of the labor protection, medical services,
Third, the social life of labor security, medical services, education, safety and other needs *** to solve the demand;
Fourth, the production and operation of enterprises, the development of the environment and other aspects of the need to *** coordinated to solve the demand;
Fifth, on the *** department and its staff, criticisms, complaints and so on.
What are the precautions for hot air ballooning
Hot air ballooning has a history of more than two hundred years in foreign countries, but it started late in China, and now there are only more than 120 hot air balloons in the country. The cost of a hot air balloon is expensive, about 80,000 yuan to 100,000 yuan each, and you need to get a Hot Air Balloon Pilot License issued by the Civil Aviation Administration of China (CAAC) to fly a hot air balloon. Each flight not only need more than ground staff to cooperate, but also to the civil aviation management department and the air force command department in advance to apply for the record. Even if you just go to the club "experience", each "free flight" 30 minutes to 600 yuan, more expensive than playing golf.
Currently in the country to obtain the qualification to drive a hot air balloon, tuition costs 16000-18000 yuan. After learning, such as professional clubs to fly, each flight still need to pay a certain flight costs; and experience the cost of flying an hour about 400-500 yuan; if you want to buy a hot air balloon, in their own areas to engage in flight activities, but also need to be in the air traffic control department for the flight of airspace approval and declaration, to buy a standard hot air balloon need to spend 76,000 yuan. The cost of buying a standard hot air balloon is about 76,000 yuan. In some tourist attractions in China, there are "tethered flight", that is, fixed not to fly away from the hot air balloon, although it can only rise to a height of 30 to 50 meters, but still can be a preliminary experience of hot air balloon flight "King of the world" feeling.
Precautions:
1, in order to prevent fire, the flight crew must wear cotton fabric clothing, can not wear skirts, high heels, sandals, etc., must wear sports shoes. And in order to prevent burns, must be wearing a long coat and long pants, wearing a cotton baseball cap.
2, high blood pressure, heart disease patients can not carry out hot air ballooning.
3, hot air balloon flight is the best time in the sunrise within two hours or two hours before sunset, because the time period the most stable airflow. The windy season is not suitable for hot air ballooning.
4, each flight has a certain flight cost, basically every hour cost 400 to 500 dollars.
5. The service life of a balloon is basically 9 years, and annual and sequential inspections are required.
6, high-altitude flight should pay attention to the cold, every 1000 meters, the temperature drops 6.5 degrees.
7. Professional flights require a driver's license issued by the Directorate General of Civil Aviation.
8, in addition to the necessary equipment, you need to wear a GPS global positioning system, electronic compass, walkie-talkie, utility knife, ZIPPO lighter, etc.
What are the precautions for RT-PCR?Some good habits in the experiment
Before adding reagents, mix it a little bit, so as not to leave it for a long time uneven concentration
Pipette gun should be returned to the position of the maximum measurement after use, to prevent the spring from losing its elasticity over time
Always remember to turn off the water bath box, remember remember remember remember
Discuss more and more with people, and at the same time pay more attention to other people discussing the experience. This is almost the fastest way to improve
Prepare all the reagents by yourself, so that you can find the cause of the problem
Measures to prevent contamination of RNAase
1. All glassware should be dry-roasted at 180℃ for 6hrs or more before use.
2. Plastic containers can be soaked in 0.1% DEPC water or rinsed with chloroform (Note: Plexiglas containers can be corroded by chloroform, so they cannot be used).
3. Plexiglass electrophoresis tanks, etc., can be washed with detergent, rinsed with double-distilled water, ethanol drying, and then immersed in 3% H2O2 at room temperature for 10min, and then rinsed with 0.1% DEPC water, drying.
4. The prepared solution should be treated with 0.1% DEPC as much as possible, at 37℃ for more than 12hr. Then autoclave to remove residual DEPC. reagents that cannot be autoclaved should be prepared with DEPC-treated sterile double-distilled water, and then filtered through a 0.22μm membrane to remove bacteria.
5. Operators wear disposable masks, hats, gloves, gloves should be changed frequently during the experiment.
6. Set up a special laboratory for RNA operation, and all instruments should be specialized.
II. Commonly used RNAase inhibitors
1. Diethyl pyrophosphate (DEPC): It is a strong but incomplete RNAase inhibitor. It inhibits enzyme activity by denaturing the protein by binding to the imidazole ring of histidine, the active group of RNAase.
2. Guanidine isothiocyanate: Currently considered the most potent RNAase inhibitor, it inactivates RNAase while cleaving tissue. It both disrupts cell structure to dissociate nucleic acids from nucleoproteins and has a strong denaturing effect on RNA enzymes.
3. Oxovanadium ribonucleoside complex: A complex formed by oxovanadium ions and ribonucleosides, which combines with RNAase to form a transition state-like substance that almost completely inhibits RNAase activity.
4. Protein inhibitor of RNAase (RNasin): an acidic glycoprotein derived from rat liver or human placenta, RNasin is a non-competitive inhibitor of RNAase that binds to and inactivates a wide range of RNAases.
5. Others: SDS, urea, diatomaceous earth, etc. also have a certain inhibitory effect on RNAase.
Because DEPc does not selectively modify proteins and RNA, it cannot be used in the isolation and purification of RNA, and it is not compatible with some buffers (e.g., Tri)
In all RNA experiments, the most critical factor is the isolation of the full-length RNA. and the main reason for the failure of the experiment is the contamination of ribonuclease (RNAase).
RNA enzymes can tolerate a variety of treatments without being inactivated, such as boiling and autoclaving.
Contamination by researchers The most significant potential source of contamination of RNA enzymes is the hands of researchers. Therefore, gloves should be changed frequently when conducting RNA experiments.
Precautions for wide-mouth vials, precautions for fine-mouth vials, what are the uses and precautions for funnels?Fine Mouth Bottle
A glass container used for storing liquid reagents, hence the smaller mouth. There are two kinds of transparent and brown, the brown bottle is used to hold reagents that need to be stored away from light.
Precautions for use: not for heating. When you take the reagent, the stopper should be placed upside down on the table, and after use, add a stopper to tightly plug, and seal if necessary. Because the inside of the mouth of the bottle is frosted, and the glass frosted stopper matching, and therefore can not hold strong alkaline reagents. If the alkaline reagent, to use rubber stopper.
Wide-mouth bottles
Wide-mouth bottles are glass containers used to hold solid reagents, there are two kinds of transparent and brown, brown bottles are used to hold reagents that need to be protected from light.
Precautions for use: Cannot be used for heating. When you take the reagent, the stopper should be placed upside down on the table, and after use, add a stopper to tightly plug and seal if necessary. Because the inside of the mouth of the bottle is frosted, with a glass frosted stopper matching, so the glass stoppered wide-mouth bottle can not hold strong alkaline reagents. If the alkaline reagent, to change the rubber stopper
What are the precautions of the loanLoan through the China Merchants Bank, the loan amount is small (less than 50,000), such as you have been a China Merchants Bank users, and downloaded the China Merchants Bank mobile banking APP, you can try to log in to the mobile banking, click on the "my" - "All" - "Loans" - "I want to borrow money", and try to apply through this interface. If you meet the conditions of the credit card, you can try to apply for a credit card.
If the loan amount is more than 50,000 yuan, if your city has a China Merchants Bank, you can try to apply for a loan through the China Merchants Bank outlets, due to the different conditions required for each loan program and the application materials, the current use of the loan is as follows: the purchase of a car, the purchase of a car parking space, renovation, education, education and learning funds, bulk consumer shopping, travel and other personal or family legal consumption; and business purposes of the turnover. You can call 95555 from 8:30 to 18:00 to enter the manual service, provide the purpose of the loan and the city to learn more about the required information. Please refer to the comprehensive review result of the personal loan department of the bank where the loan application is approved or not.
What are the precautions for soldering1, tin furnace setting temperature should not be too high to avoid accelerated oxidation of the tin surface. Generally set at about 300 degrees or according to the actual use of the need to set;
2, the first appropriate amount of tin in the tin pot, connected to the power supply, turn on the power switch, adjust the temperature to 250 degrees or so, with the soldering bar has been red heating tube coated with tin, to the tin surface to cover the heating tube. When the tin began to melt, it should be timely to add into the tin until the surface of the melted tin to the appropriate height, in the tin furnace is not welded tin, do not electrify the heating.
3, the general temperature range of the tin furnace: 0-500 degrees. But the actual maximum temperature in: 300-400 degrees or so;
Attachment tin furnace when using the precautions
A. Tin furnace connected to the ground, please make sure that the user must be connected to the use, and ensure that the ground is good, in order to plan for the safety of;
B. Tin furnace should be checked before use of the power supply voltage match;
C. Tin furnace should be kept dry, do not work in the wet or rainy conditions;
C. Tin furnace should be kept dry, do not work in the damp or rain environment;
D. Tin furnace should be kept dry, not in the wet, not in the wet or rainy conditions. Work;
D. Tin furnace should be placed in a stable, around 0.5m range can not be placed within the flammable and other items;
E. The operator should use goggles and heat-resistant gloves, the use of attention to avoid the fall of foreign objects into the melting tin pot, to prevent accidents;
F. After the power is forbidden to move, can not be arbitrary knocking, dismantling and installation of its electric parts;
G. Tin furnace should be kept dry before use, do not wet or rainy environment;
G. When using the shell has a temperature of 50 ℃ -80 ℃, this is a normal phenomenon, pay attention to the high temperature, do not touch the shell;
H. Use should be turned off after the completion of the power in the case of unattended, do not energize the heating;
I. Such as failures, should be asked to have a professional maintenance skills of the personnel to check the elimination.
What are the precautions for blood transfusion?(1) Before transfusion, the appearance of whole blood must be strictly inspected, and the blood bag must be inspected to see if there is any damage or leakage, and whether the color of the blood is qualified; the patient, the cross-compatibility report form and the blood to be transfused must also be carefully checked to see if there is any error between the patient, cross-compatibility report form and the blood to be transfused, including the patient's and the donor's name, gender, ABO and Rho (D) blood type, the result of cross-compatibility test and the antiglobulin test, the number of the bag, the blood type and the volume of the blood, etc., and should be There should be two persons to check the accuracy before blood transfusion. (2) When transfusing blood, one should go to the patient's bedside to check the case number, patient's name, blood type, etc., and after determining that the recipient is himself/herself, the transfusion should be carried out with a standard transfusion device equipped with a filter (the pore size of the filter mesh is about 170 micrometers, and the total effective filtration area is 24-34 cm2, which can filter out the aggregated platelets, leukocytes, and fibrin that may be present in the blood and blood component products). (3) Blood is removed from the cooler again before transfusion and should not remain at room temperature for more than 30 minutes. Gently mix the blood in the blood bag before transfusion and avoid violent shaking. No other drugs should be added to the blood; if dilution is needed, only saline for intravenous injection should be used. (4) Use saline to flush the blood vessel before and after transfusion. In case of continuous transfusion of blood from different blood suppliers, after the previous bag of blood has been transfused, the transfusion vessel should be flushed with intravenous saline before the next bag of blood is received for further transfusion. (5) The process of blood transfusion should be slow and then fast, and then adjust the speed of transfusion according to the condition and age, and closely observe the recipient for any adverse reaction to blood transfusion, and deal with it in time if there is any abnormality. During the initial 10-15 minutes of transfusion or the first 30-50 ml of blood, the recipient should be closely watched by the medical staff for any adverse reaction. If any adverse reaction occurs, the transfusion should be stopped immediately and reported to the physician in charge for prompt treatment, and at the same time notify the Transfusion Department or the blood bank for necessary investigation. Usually, it is not necessary to warm the blood for transfusion. (6) After transfusion, the blood bag should be kept in the refrigerator at 2-8℃ for 24 hours for verification in case of accidents. (7) Upon completion of blood transfusion, the medical staff will fill in the survey report of blood transfusion reaction item by item and return it to the Blood Transfusion Department for retention on the second day after completion of blood transfusion. The blood transfusion department will report the statistics to the medical office (section) every month, and the physician in charge will record the blood transfusion situation in the medical record. (8) After the completion of blood transfusion, the medical staff will be transfusion single second link posted in the medical record.
What are the precautions for reversing?When backing up, try to turn the front of the car toward a place with open sight lines and room to maneuver in order to facilitate observation of moving people or cars. Don't back up in places that are not allowed to do so by traffic regulations to prevent accidents.
Expansion:
1, Introduction:
The behavior of motor vehicles moving backward is reversing. Also refers to people traveling in the middle of the "reverse", that is, from one car to another.
2, the basic essentials
Into the parking lot parking, first of all, to visually inspect the width of the parking space, there are no obstacles behind, on both sides of the car is not parked, the length of the car is consistent with their own models. When you can't see at night, it's best to come down and observe the exact position before pouring.
Backing up as much as possible from the left side of the reverse (in the case of conditions permitting). The left side is easy to reverse people to observe, in the process of reversing, to look behind first, and then pay attention to both sides of the rearview mirror. The lateral distance on the right side is somewhat greater than on the left. Because the turning radius of the front wheels is larger than that of the rear wheels, the front end will be thrown outward when reversing, and it is easy to scrape the right side without paying attention to this. The speed in reversing is mainly idling, and the body is corrected in time. Each car's reference point is different, the timing of the direction is different, here is not a generalization. [1]
Sometimes you will be parked along the shoulder in a straight line, and often you will have to back into the position between the two cars that have been parked. This kind of reversing is difficult, and it is best to have someone on hand to guide you. When backing up, pay attention to the lateral distance from the neighboring car. From the rear-view mirror to see the rear of the car almost to the shoulder of the road when the direction of the rapid enough, such as the body of a positive quickly back to the direction of the car, do not forget to reverse with the distance between the car behind. Don't forget the distance between you and the car behind you. If you can't grasp the distance between you and the car, come down and look at it before you reverse. In the process of backing up the direction of the speed of fast, in order to put the body in a short period of time. Sometimes a time forward, reverse may not put the body, you need to repeat a number of times before the car will be parked.
What are the precautions for shooting?The more classic positions for shooting are prone, standing and kneeling, and squatting can be used in special circumstances. Here is a brief introduction to an exercise, prone position on the hundred meters of immobile target shooting essentials.
I. Posture.
The shooter lying prone on the shooting mat to take a good preparatory position, its torso and shooting to the projected angle is generally 10 to 20 degrees. Feet to become "eight" close to the ground, the left leg straight and the left side of the body is similar to a straight line. The angle between the left forearm and the ground should not be less than 30 degrees, and the angle between the upper arm and the ground should be maintained at about 45 degrees. The bottom of the butt of the gun rests against the right shoulder socket, against the shoulder should be against the solid, close to the clavicle, the right hand grips the handle (neck of the gun). The right cheek is pressed tightly, with the gravity of the head squarely downward when the cheek is pressed, and the neck relaxed. The entire body empowered by the gun can only feel the force forward, downward, without lateral push, pull force.
The height of the support depends on the height of the individual and the length of the right arm, generally in the 20 - 26 centimeters or so is more appropriate.
Second, according to the gun.
According to the gun, the right side of the body and the gun slightly into a line, the right hand tiger's mouth forward to grip the gun handle, the first section of the index finger on the board machine, the right elbow as far as possible in the ground with the front brace. Hold the magazine in the left hand and brace the left elbow on the ground, keeping both elbows steady. Chest up, the body slightly forward heel (right elbow does not leave the ground), the upper body naturally collapsed, the two hands remain unchanged, so that the butt of the gun is really against the shoulder socket, the face is slightly forward, naturally close to the cheek.
The main points of the gun can be summarized in ten words, namely: positive, grip, against, fixed, collapse, not top and not pull.
Positive: the right side of the body and the gun body is basically square, the gun surface should be positive.
Grip: the right hand tiger's mouth on the positive grip grip, to keep the grip unchanged when firing, the right wrist should be inwardly closed, collapsed, and held up.
Against: against the shoulder position of the appropriate height, close indeed.
Fixed: Both elbows should be fixed.
Collapse: the upper body naturally collapsed, to maintain a solid position according to the gun.
Not topping and not pulling: After completing the gun action, keep the force unchanged, not to pull the gun backward and forward shoulder.
Third, aiming.
According to the gun, the upper edge of the gap, the upper edge of the collimator and the lower edge of the target in the same straight line. If not pointing to the target, can not be accommodated and twist the body or change the action according to the gun, should adjust the posture or correct the support, corrections, you can move the body left and right or the two elbows, corrections to the height of the body can be moved back and forth to refurbish the body or the two elbows inside and outside the open, but also appropriate adjustments can be made to the support. Aiming eyes should look at the target, if you stare at the collimator may make the target false, collimator in your line of sight should be the edge of a clear and sharp black shadow.
Fourth, firing.
The first joint of the index finger of the right hand is even and straight to the back of the trigger (the inside of the index finger and the gun should have a small gap), the rest of the finger strength remains unchanged. When the line of sight is close to the point of aim, start to pre-compress the trigger and slow down your breathing. When the line of sight is pointing toward the point of aim, stop breathing and continue to increase the pressure on the trigger until the shot is fired. Correct and consistent aim should be maintained at the moment of firing. If the line of sight deviates from the point of aim or if you cannot continue to stop breathing, you should neither increase nor relax the pressure on the trigger, and then continue to withhold pressure on the trigger after correcting or changing your breath. Until the gun goes off, complete the shooting. This is also commonly referred to as: "conscious firing, unconscious gun".
V. Problems that can easily exist.
1, fierce trigger. Easily caused by the gun body swing, most of the projectile is off to the left or off-target, the cause of the violent deduction of the board machine is due to the shooter's nervousness to capture the aiming point and cause. This is the weakness of the failed shooter.
2, the timing of the shot is not good. Performance is either too early or too late, often miss the time, firing more hesitant.
In addition, when shooting, it should be the main focus on the collimator, notch on the relationship between the level, always keep the collimator and notch level, that is to say, when shooting, it should be to look at the collimator, notch clear and look at the target fuzzy.
Above, just shooting some of the basic essentials, affecting the accuracy of shooting for a number of reasons, as well as the wind, sunlight and other reasons, but as long as it is mastered the correct shooting essentials, or can be overcome.
What are the precautions for briefing?A briefing is an official document in which a superior informs a subordinate about a person or event. Circulars have a wide range of applications and can be used by party and government organizations and units at all levels. Its role is to praise the good and good deeds, criticize the error and the evil spirit, notify the evil accidents that should be warned, convey the important situation and the need for all units to know the matter. Its purpose is to exchange experience, learn lessons, educate cadres, workers and the public, and promote the further development of work. Commendation (criticism) of the body of the circular structure has three parts: the first part, explain the reasons for recognition or criticism, that is, to write the advanced deeds or the wrong facts after the situation, the requirements of the narrative approach to truly and objectively reflect the facts; the second part of the facts of the description of an accurate analysis of the facts, pertinent evaluation, so that it does not exaggerate, do not narrow, so that people can be inspired by the good people and things from the mistakes in the lessons to learn; the third part, generally on the commendation of the facts of the case of the incident. The third part of the briefing is generally to commend the advanced or criticize the mistakes to make awards or punishment. Finally, according to the briefing, for the reality of the need to issue a call or put forward requirements. The body structure of the briefing generally has two parts: one is notified of the situation, the second is the hope and requirements.