Answer:
Kaifeng Qixian People's Hospital
Nucleic acid test
Collection time for the morning: 8:00 - 12:00 and afternoon: 14:00 - 17:00.
Kaifeng Qixian People's Hospital is located in Qixian Beihuan Road, No. 10 outpatient clinic, the collection site in the door of the infection department of the Qixian People's Hospital Xiguan Hospital District. You can call 0371-28973913
New coronavirus nucleic acid detection principle
The most common method of detecting specific sequences of the new coronavirus is fluorescence quantitative PCR. because the PCR reaction template is only DNA, so before the PCR reaction, the new coronavirus nucleic acid should be reverse transcribed into DNA. in the PCR reaction system, which contains a pair of specific primers. the PCR reaction system contains a pair of specific primers as well as a Taqman probe, which is a specific oligonucleotide sequence labeled with a reporter fluorescent motif and a quenching fluorescent motif at each end. When the probe is intact, the fluorescent signal emitted by the reporter group is absorbed by the quenching group; if the target sequence exists in the reaction system, the probe binds to the template during the PCR reaction, the DNA polymerase degrades the probe enzymatically along the template utilizing the exonuclease activity of the enzyme, and the reporter group separates from the quenching group to emit fluorescence. For each DNA strand amplified, one fluorescent molecule is produced. The number of cycles in which the quantitative fluorescence PCR instrument is able to monitor the fluorescence to reach a pre-set threshold is related to the concentration of viral nucleic acid, the higher the concentration of viral nucleic acid, the smaller the Ct value. Different manufacturers' products will determine the positive judgment value of the product based on the performance of their own products.