We mainly sell fluorescence quantitative pcr, our engineers are more professional, you can describe the problem in detail, I ask our engineers. You can describe the problem in detail, I ask our engineers. You can also direct my name, I will solve as soon as possible. You can also read the attached file.
ABI?7500 Fluorescence PCR Instrument Technical Parameters
A? Technical parameters
1.5-color fluorescence can be detected at the same time for multiple PCR detection
*2?Excitation light source: tungsten halogen lamp, service life is not less than 2000 hours, the software for real-time monitoring of the light source,
*3?Detector: Cold CCD imaging system, simultaneous imaging of the 96-well plate without time lag, to ensure the accuracy of the results
4 Temperature control system: semiconductor temperature control
*5?Temperature accuracy ≤ ± 0.05 ℃ (95 ℃), ≤ ± 0.25 ℃ (35 ℃ - 95 ℃ range)
*6?Temperature control range: 4 ℃ ~ 100 ℃
7?Kinetics of the linear range: 9 orders of magnitude, the distinction between 5000 and 10,000 copies of the twice the difference in the concentration of The confidence level is as high as 99.7%
**8?The software supports ROX or other dyes as reference fluorescence, which can be used to eliminate spiking errors and monitor the reaction system for evaporation. At the same time, the software supports the setting of no reference dye
9?Open reagents, open consumables, support for 0.2ml 96-well plates, 8 tubes and single tubes
**10The performance of the instrument has been verified by the original factory-installed kits: 4 replicates of each of the initial template standards, which can discriminate between 1,250, 2,500, 5,000, 10,000 and 20,000 copies, to verify the accuracy of the linearity. The 36 replicates of 5000 copies and 36 replicates of 10000 copies can be distinguished with 99.7% confidence
*11?Throughout the entire experimental process, the excitation light source, the detector do not need to move
12?Sample volume: 10-100 μl (20-50 μl is recommended)
13?Sensitivity: the ability to detect single-copy human genome genes in the human genome. Single-copy human genome genes in the human genome.
14?Excitation/emission filters: Contains 5 sets of pre-loaded filters, supports FAM/SYBR?GREENHEX/TET/VIC/JOE, TAMRA/Cy3, Texas?Red/ROX, Cy5 dyes, and the software supports customers to add their own dyes.
*15Can detect 450~750nm continuous wavelength
16?Data analysis: 5-color fluorescence PCR data can be analyzed at the same time; absolute quantification and relative quantification functions; multiple watchdog genes can be selected for data processing; data can be further processed automatically according to the amplification efficiency to get more accurate and reliable results; the software supports analyzing an unlimited number of The software supports the analysis of unlimited number of data files to increase the reaction throughput; it has the functions of allele and melting curve analysis; the software supports the statistics of spiking replicates and biological replicates.
*17. Configure professional primer probe design software for gene primer probe design, and ensure the consistency of annealing temperature.
*18. With medical device registration certificate