What are the precautions for using the pipette?

1. Setting the pipette volume \x0d\ Adjusting from a large volume range to a small volume range is the normal method of adjustment, and can be done by rotating the scale counterclockwise. When adjusting from a small to a large volume range, you should first adjust it to exceed the set volume scale, and then adjust it back to the set volume, which ensures the accuracy of the pipette \x0d\ 2. Assembling the Pipette Gun Tip \x0d\ Insert the pipette gun vertically into the tip, rotate it left and right half a turn, and then tighten it. The method of hitting the tip with the pipette is very undesirable. Long-term operation of this kind leads to the loosening of the pipette parts due to impact, which can seriously lead to the jamming of the knob for adjusting the scale\x0d\ 3. Suction and release of liquid, vertical pipette tip immersed in the liquid surface of the tip of the tip of the pipette is less than 3mm, and the tip of the tip of the pipette before suction is pre-lubricated with the liquid. Slowly suction, slowly release and release the liquid, and if the amount is small, the tip of the tip of the tip of the pipette should be relied upon to the inner wall of the container\x0d\ 4. The pipette gun should not be placed flat, the liquid in the tip can easily contaminate the inside of the gun and may cause the gun's spring to rust\x0d\\x0d\ 5. The pipette gun should be scaled to the maximum after each experiment to allow the spring to return to its prototype in order to prolong the service life of the gun\x0d\\x0d\ 6. When aspirating liquids, be sure to release your thumb slowly and steadily, and never allow it to be released suddenly to prevent the solution from being sucked in too quickly and washed into the pipette gun. Inhale too quickly and rush into the liquid extractor to corrode the plunger and cause leakage. \x0d\ 7. In order to obtain a high degree of accuracy, the pipette tip should be used to aspirate the sample solution once before formal pipetting, because when aspirating serum protein solutions or organic solvents, a layer of "liquid film" will remain on the inner wall of the pipette tip, which will cause a small amount of liquid to be discharged, resulting in an error. \x0d\ 8. Concentration and viscosity of the liquid, will produce errors, in order to eliminate the amount of error compensation can be determined by the test, the amount of compensation can be used to adjust the knob to change the number of readings in the reading window to set. \x0d\ 9. Analytical balances can be used to calibrate the pipette by weighing and calculating the weight of the pure water taken. 1mL of distilled water at 20°C weighs 0.9982g. Do not rotate the buttons out of range within the pipette's range, as this will jam the mechanism and damage the pipette. \x0d\ 10 When setting the range, please note: the numbers are clearly in the display window, rotate to the desired range \x0d\ 11. The pipette is strictly prohibited to draw up liquids with strong volatility and corrosiveness (e.g., concentrated acid, concentrated alkali, organic substances, etc.). \x0d\ 12. Strictly prohibit the use of pipette blowing mixed liquids. \x0d\ 13. Do not use a large-range pipette to pipette a small volume of liquid, so as not to affect the accuracy. At the same time, if you need to pipette a large amount of liquid outside the range, please use a pipette to operate.