The precautions to be taken during the use of pipettes are as follows:
I. Setting the volume of pipette: 1. When adjusting from a large volume to a small volume, it is the normal adjustment method, rotating the scale counterclockwise; 2. When adjusting from a small volume to a large volume, it can be adjusted clockwise to the scale that exceeds the set volume, and then adjusted back to the set volume, which can ensure the best accuracy.
II. Assembling pipette tips: 1. For single-channel pipettes, insert the end of the pipette vertically into the tip, turn it slightly from side to side, and tighten it. 2. The method of tightening the tip by repeatedly hitting it with the pipette is undesirable, as such operation will cause the pipette parts to loosen due to strong impacts, and in serious cases the knob of the adjusting scale will be stuck. 3. For multi-channel pipettes, align the first channel of the pipette with the first tip, insert it at an angle, and shake it back and forth slightly to tighten it. Shake it back and forth slightly to tighten it, and insert the tip slightly over the O-ring.
Three. Conservation: 1, such as liquid accidentally into the piston chamber should be removed in a timely manner pollutants; 2, the pipette after use, the pipette range to the maximum value, and will be placed vertically in the pipette rack; 3, according to the frequency of use of all the pipettes should be cleaned with soapy water on a regular basis or with 60% of the disinfection of isopropyl alcohol, and then cleaned with double-distilled water and air dry; 4, to avoid placing in the temperature at a higher place in order to prevent deformation of the leakage of liquid or Not allowed; 5, found that the problem in a timely manner to find a professional to deal with; 6, when the pipette nozzle liquid do not pipette horizontal or inverted placed to prevent the liquid into the piston chamber corrosion of the pipette piston; 7, the correct use of the pipette suction, discharge, in order to achieve a high degree of accuracy; 8, usually check whether the leakage of liquid method: suction after the liquid in the liquid stopped for 1-3 seconds to observe the pipette tip within the liquid level is declining; if the liquid level falls first Check whether there is a problem with the tip, if there is a problem to replace the tip, after replacing the tip, the liquid level is still falling that the piston assembly has a problem, you should look for professional maintenance personnel to repair. 9, need high temperature disinfection of pipettes should be the first to check the use of pipettes suitable for high-temperature disinfection and then processing.