To improve the efficiency of membrane transfer (my experiment requires that the more proteins transferred to the membrane, the better, no matter what size proteins) I don't know what are those ways?

No matter how to transfer, there will be the problem of incomplete protein transfer (too small voltage too short time) or excessive transfer (too large voltage too long time), the fish (small molecular weight proteins) and the bear's paw (large molecular weight proteins) can't be both hehe. It is suggested to cut the gel into two halves, for example, with 35KD as the boundary, and transfer the membrane separately, the lower half of the time is short, and the upper half of the time is a little longer, it should be better. Our lab has been using the Beijing Saizhi's semi-dry transfer meter, the effect is good, you can try.