The detection process of fecal coliforms in cosmetics testing projects:
10g/mL sample + 90mL sterile saline → 10mL + 10mL double lactose bile (including neutralizer) Culture medium → Fecal coliform culture at 44.5℃, 48h
Note: In the cosmetics testing project, if the sample contains greasy ingredients, such as essential oils, liquid paraffin and Tween need to be added in the sample preprocessing 80. Homogenize with physiological saline so that the sample can be evenly dispersed.
What kind of microorganism is fecal coliform? Let’s introduce it in detail below:
Coliform: Coliform is not a bacteriological classification name, but a term in the field of health bacteria. It does not represent a certain type or genus of bacteria, but refers to A group of bacteria associated with fecal contamination that have certain properties that are not identical biochemically and serologically.
Definition (GB2010): Aerobic and facultative anaerobic Gram-negative bacilli that can ferment lactose, produce acid and gas under certain culture conditions.
Fecal coliform: a type of coliform, also known as heat-resistant coliform. Culture temperature: 44.5±0.5℃. Fecal coliform is a must-detect microbial item in cosmetics testing projects.
Escherichia coli: (Escherichia. coli) is commonly known as Escherichia coli. Pathogenic Escherichia coli is divided into 5 categories according to different biological characteristics:
Pathogenic Escherichia coli Pathogenic Escherichia coli (EPEC),
Enterotoxigenic Escherichia coli (ETEC),
Enteroinvasive Escherichia coli (EIEC),
Enterohemorrhagic Escherichia coli Escherichia coli (EHEC),
Enteroadhesive Escherichia coli (EAEC)
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