The thermal sterilization method needs to reach a certain heating time under the specified sterilization conditions such as temperature and pressure. Comprises the following steps: ① flame sterilization; ② Dry heat sterilization; ③ autoclaving; ④ Circulating steam sterilization; ⑤ Boil and sterilize.
2, filtration sterilization method (physical sterilization)
Filter sterilization is a common sterilization method to remove microorganisms by physical means such as screening or filter material adsorption. Suitable for articles that cannot be heated, such as culture medium, test solution and liquid medicine containing soluble or unstable substances. The biggest disadvantage of filtration method is that it can't filter out viruses.
3, radiation sterilization method (physical sterilization)
(1) radiation sterilization is a method to kill microorganisms by using radiation generated by radioactive sources including radioisotopes, and it is suitable for radiation-resistant articles such as glass products, magnetic products, metal products, rubber products, plastic products and fiber products.
② Ultraviolet sterilization is a method of killing microorganisms by ultraviolet irradiation, which is suitable for glass products, metal products, rubber products, plastic products and fiber products, and can also be used for facilities, equipment, water or medicines.
4, gas sterilization method (chemical sterilization)
Gas sterilization is a method of using ethylene oxide or formaldehyde to kill microorganisms, which is mainly used in glass products, magnetic products, metal products, rubber products, plastic products, fiber products and so on. , can also be used in facilities, equipment or powdered drugs. When gas sterilization is used, as a prerequisite, the sterilized items will not deteriorate.
5, liquid medicine sterilization method (chemical sterilization)
Commonly used liquid medicines include ethanol, cresol, phenol water or formalin water. , suitable for glass products, magnetic products, metal products, rubber products, plastic products, fiber products and other items, but also for fingers, aseptic boxes or aseptic equipment.