Silk protein (Fibroin; シルクタンパク ) also known as: silk protein. Silk protein, is a natural polymer fiber protein extracted from silk, the content of about 70% to 80% of the silk, containing 18 kinds of amino acids, of which glycine (gly), alanine (ala) and serine (ser) accounted for about 80% of the total composition.
On November 20, 2014, the State Key Laboratory of Genome Biology of the Domestic Silkworm at Southwest University obtained empty silk glands by knocking out the Fib-H gene, and the silkworm spit out synthetic silk protein, which was the first time at home and abroad.
Question 2: What is the effect of using silk in skin care products? Silk cosmetics bio-activity is very good, biocompatibility recognized by the medical community, only need water, after a special biological process of silk natural state will be hydrolyzed to release silk protein. Basically, the market will be more silk cloth, can use silk skin care brands are usually high-end market, relative R & D strength and production strength are relatively large.
Question 3: Palmitoyl Hydrolyzed Silk Protein What is Palmitoyl Hydrolyzed Silk Protein Basic Information
Chinese Name: Palmitoyl Hydrolyzed Silk Protein
Chinese Synonym: Palmitoyl Hydrolyzed Silk Protein
English Name: PALMITOYL HYDROLYZED MILK PROTEIN
English Synonym: PALMITOYL HYDROLYZED MILK PROTEIN
Formula: C16H31O *
Molecular Formula: C16H31O *
Molecular Weight: 239.41674
CBNumber: CB8962706
Question #4: What is the special ingredient of the national soap? Snow lotus? Hydrolyzed silk Yes, there are pure plant extracts. After use makes the face not tight, anti-inflammatory and calming, reduce acne pimples.
Question 5: How to prepare silk protein hydrolyzed liquid? The more detailed the better, thank you very much! 1 Materials and methods
1.1 Experimental materials
1.1.1 Raw and auxiliary materials
Silk, Pueraria Mirifica, Citrus aurantium berries, umeboshi are purchased from Xuzhou market. Sucrose, neutral protease (130,000 u/g), trypsin (4,000 u/g) were provided by the laboratory, and papain (650,000 u/g) was provided by Guangxi Haifa Biological Enzyme Products Factory.
1.1.2 Main reagents
Formaldehyde reagent, concentrated sulfuric acid, concentrated hydrochloric acid, sodium hydroxide, anhydrous sodium sulfate, anhydrous calcium chloride, chlorine, potassium hydrogen phthalate, methyl red, copper sulfate, boric acid, beef paste, peptone, agar, table salt, 731 and 723 anion and cation exchange resins, and the mixture of reminder: 1 volume of methylene blue and 2 volumes of methyl red indicator. mixture.
1.1.3 Main equipment
Electrothermal constant temperature drying oven, electrothermal constant temperature incubator, multifunctional pulverizer, low-speed large number of centrifuges, rack-and-disk balance, magnetic stirrer, precision pH meter, electronic balance, digital thermostatic water bath, portable pressure steam sterilizer, electromagnetic furnace.
1.2 Experimental Methods
1.2.1 Process Flow
(1) The process flow of beverage blending using acidolytic solution
Silk degumming→acid digestion→cation exchange resin deacidification→silk pigment amino acid
Chinese herbal medicine→cleaning→leaching→collectivation→coarse filtration→centrifugation Blending→
Flavoring, VC, sucrose, etc.
Filling → Sealing → Sterilization → Cooling → Sensory evaluation and hygiene testing → Finished products
(2) The process flow of beverage blending using enzymatic solution
Silk degumming → CaCl2 dissolution of silk pigment → Enzymatic → Enzyme inactivation → De-CaCl2 → Silk pigment amino acids
Chinese herbs → Cleaning → Extracting → Coarse filtration → Centrifugation → Blending → Filling
Flavoring, VC, Sucrose etc.
The flavor of the product is not only the same as the flavor of the product, but also the taste of the product. VC, sucrose, etc.
→ sealing → sterilization → cooling → sensory evaluation and hygiene testing → finished product
1.2.2 Methods of silk degumming
The washed and dried silk was boiled in Na2CO3 solution (0.4%, 0.5%, 0.6%) for treatment (20min, 30min, 40min) to determine the optimal degumming conditions.
1.2.3 Methods of acid digestion of silk pigment
The silk pigment after degumming was dried to do a one-way experiment. First selected acidity of 3M, solid-liquid ratio of 1:50, acid digestion at 111 ℃ for 15h, the determination of amino acid content per hour, can get the appropriate range of acid digestion time; and then selected solid-liquid ratio of 1:50, the acid digestion time in the above range, 110 ℃ to take 1.0M, 1.5M, 2.0M, 2.5M, 3.0M, 3.5M, 4.0M, 4.5M, 5.0M, etc. different concentrations of acid digestion, can be selected as appropriate. concentration acid digestion, can be selected suitable acid concentration range; finally selected acid concentration and time in the appropriate range, by selecting different solid-liquid ratio of 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:80, to determine the most suitable range of solid-liquid ratio. The obtained acid concentration, solid-liquid ratio, acid digestion time were selected in the appropriate range of three levels to do three factors and three levels of orthogonal experiments, so as to find out the optimal acid digestion conditions.
1.2.4 Dissolution of silk pigment in CaCl2
Different concentrations of CaCl2 (30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%,) were prepared and the dissolution of silk pigment was measured to find out the optimum concentration of CaCl2 for dissolving silk pigment.
1.2.5 Research methods of enzyme digestion process (1) Optimal hydrolysis enzyme selection. Make the silk protein liquid in three kinds of protease optimal enzyme amount, enzyme temperature, enzyme pH and the same enzyme time for enzymatic hydrolysis, compare the three kinds of enzyme enzyme hydrolysis effect, according to the rate of amino acid yield selected hydrolysis effect of the best enzyme. (2) Determination of enzymatic conditions. According to the above experiments to determine the best filipin proteinase, respectively, the substrate concentration, pH, temperature to determine the three levels of orthogonal experiments, in order to determine the optimal conditions for enzyme digestion. The substrate concentration was 4%, 5% and 6%, pH was 5.0, 5.5 and 6.0, and the enzymatic temperature was 50°C, 55°C and 60°C, respectively.
1.2.6 Acid solution deacidification
The acid solution was deacidified with 732 strong acid cation exchange resin, and when the effluent made ninhydrin purple, the column was stopped, and the ion-exchange column was washed with deionized water until the effluent was nearly neutral, and then the amino acids were eluted with 0.5M ammonia until the ninhydrin didn't make it purple.
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Question six: hydrolyzed silk have after-sun repair with aloe vera gel or flower water to do the mask is better, right There is a calming effect of
Question seven: what is the role of facial cleanser containing hydrolyzed silk protein It mainly depends on the concentration of silk protein and the quality of the silk protein. If it is good, the face wash will be smoother to use, nothing, the face wash is only cleaning
Question 8: What are the benefits of silk protein cosmetics? ? There are many benefits, silk protein and natural cellulose, polymers or other products mixed with silk cosmetics have a variety of excellent performance. But the premise is 100% natural silk. Real silk cosmetics bio-activity is very good, biocompatibility recognized by the medical community, only need water, after a special biological process of silk natural state will be hydrolyzed to release silk protein. Nowadays, silk protein is used as a substitute for sheep's fetus, and you can check the properties and capabilities of silk protein, which are scientifically based. The base material of Aizhi Yi silk protein cosmetics has good water solubility, can maintain the stability of the aqueous solution, no ***, no allergy to the skin, this silk gelatin coated powder has a moderate gloss, transparent, and can cover the defects of the surface of the skin very well
? such as wrinkles, pores or rough tissue. Silk's derivation in the medical field is also superb , it's just that people who don't study this in life, rarely know about it.
Question 9: What are the effects of silk protein? Specific point 50 points The efficacy and value of silk protein (this article has a professional silk quilt manufacturers Shao's home textile finishing to provide)
Silk protein (Fibroin; シルクタンパク ) is also known as: silk protein. Silk protein, is a natural polymer fiber protein extracted from silk, the content of about 70% to 80% of the silk, containing 18 kinds of amino acids, of which glycine (gly), alanine (ala) and serine (ser) accounted for about 80% of the total composition. Overview
Slow-release, etc., and can be obtained after different treatments in different forms, such as fibers, solutions, powders, films, and gels. Silk protein skin care
Shelf rate, to help repair damaged skin tissue, so that the dull and tired skin to add vitality, so that in a very short period of time to restore whitening,
quality, containing proteins are much higher than the pearl, which contains nitrogen dozens of times more than the pearl, the main amino acid content of 10 times higher than the processing of natural silk refined into a natural silk protein hydrolysate. Silk protein hydrolyzed liquid penetration is extremely strong, coated in the skin can repair the skin has been damaged. Promote skin cell regeneration effect. Experiments have further confirmed that silk protein is more effective in inhibiting melanin production, silk amino acids can also inhibit the activity of tyrosinase in the skin, thus inhibiting tyrosinase melanin production, improve the dull skin tone from the inside out. Rich in amino acids and small molecule proteins, it is easily absorbed by the skin and provides the nutrients needed for skin whitening. Gradually, the skin regains and maintains its healthy whiteness and becomes silky smooth, glowing and attractive! It further realizes the dream of women who wish to have fairer skin.
Skin care 5 efficacy
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Silk protein fiber is a new type of functional fiber, with other fibers and processed products are irreplaceable unique performance and
incomparable vitality. After dyeing and weaving a variety of colorful silk protein fabrics, easier to sew and processed into various types of high-level clothing and used in high-grade home textile market. Silk protein fiber has the following five special effects: First, comfort. Silk protein fiber and the human body has excellent biocompatibility, coupled with a smooth surface, soft touch,
Its friction *** coefficient of the human body than other types of fibers to be much lower. Therefore, when our delicate skin with smooth and delicate
every inch of our skin.
Second, absorbent, moisture release good. Silk protein fiber is enriched with many hydrophilic groups such as amine group (-CHNH) and amino group (-NH2)
, and because of its porous nature, it is easy for water molecules to diffuse, so it can absorb water in the air or emit water and maintain a certain amount of moisture. In normal temperatures, it can help the skin to retain a certain amount of moisture, not too dry skin; in the summer wear, the body can be discharged sweat and heat quickly distributed, so that people feel cool and refreshing. It is because of this performance, so that the silk protein fiber is more suitable for direct contact with human skin. Third, good gloss. Silk protein fiber contains silk protein, is extracted from the snow-white silk spit out by silkworms, for the pure natural products, woven fabrics contain silky luster, after wearing a radiant. Fourth, anti-ultraviolet, heat fastness is good. Tryptophan and tyrosine in the silk protein can absorb ultraviolet rays, so silk
Protein fibers have a better anti-ultraviolet function. And because the carrier is viscose fiber, as well as the research and development process of some of the high-tech so that the silk protein fiber in the premise of anti-ultraviolet radiation, heat fastness is good, not because of the heat and sun color, so that the fabric color changes, thus reducing the aesthetic effect.
Fifth, the color is bright and good, good wrinkle resistance. Silk protein fiber in spinning and dyeing performance, with reactive neutral dyes for the best, suitable for a variety of textile products production and processing. Its woven fabrics wrinkle resistance is also significantly better than silk, silk protein fiber fabrics with good physical and mechanical properties and high stability, the fabric wet deformation is small, with good abrasion resistance.
1, deep whitening: deep into the skin layer, from the root of whitening the skin without side effects;
2, long-lasting moisturizing: NMF factor ten times more than the conventional plant or chemical moisturizers, to the skin continued to efficiently replenish water;
3, nourishing and conditioning: strong penetration, to provide the skin with the necessary nutrients, to promote the metabolism of the cells, to improve the quality of skin.
4, anti-aging living face: can activate skin cells, improve microcirculation, anti-aging wrinkles, living face;
5, antioxidant: effective resistance to external pollution, to maintain the balance of the skin's PH value, and enhance the skin's immunity
Sericulture Silk Protein Extraction Process
1, Silkworm Seed Farm chipping mouth [1] cocoon and the lower foot of the silk silk pigment proteins a hydrolysis of a filtration purification a filtrate pH test adjustment of a concentration of the silk protein. Filtrate pH test adjustment a concentration ...... >>
Question 10: I heard that the main ingredient of Weilan consistent national soap is snow lotus. Hydrolyzed silk? How much a box are handmade cold soaps, relatively speaking, the national soap will be better, goat milk soap, then just clean and moisturize, a single ingredient, the national soap, then is to the gentleman medicine production, containing hydrolyzed silk and snow lotus ingredients, snow lotus on acne acne marks also help, and make-up remover, whitening effect