High Score : Cons of Gene Chip Diagnostic Technology!!!! How comprehensive how to say! The debate needs to ask the master to help

Drawbacks: First: Biochip technology is still only in the research and development stage, and has not been transformed into a commodity for mass use, because the technology still has many problems. The main problem is the high cost, the current biochip technology requires a series of expensive cutting-edge instruments, such as photolithography machines, oligonucleotide synthesizers and laser*** focusing microscopes and other equipment, such as the Affymetrix of a set of systems need $ 13. 5 million, the ordinary laboratory for the large expenditure, is a major obstacle to the promotion of biochip technology. Moreover, sample preparation and labeling of target probes to be measured are complicated and require special technicians to operate, which all lead to relatively high research costs and cannot be popularized. Secondly, there are different degrees of false positives and differences in reproducibility in the current microarrays, which may be related to the concentration of target molecules, the concentration of probes, the sequence composition of both sides of the hybridization, the concentration of salt and temperature and other effects on the kinetics of the hybridization, so it is necessary to fully study the physical and chemical properties of these substances to improve the specificity of the chip. Thirdly, it is the standardization of microarrays, that is, how to unify and standardize the results made by different laboratories and operators, especially for those genes with low expression and insignificant differences, which are more important, and it is currently believed that these errors can be verified by traditional protein analysis techniques, such as enzyme spectroscopy, bi-directional electrophoresis, immunohistochemistry and other methods. Fourth, the resource base of genomics still needs to be greatly enriched, and the sensitivity of signal detection needs to be increased. The ultimate goal of microarray development is to realize the integration of the whole analytical process from sample preparation, chemical reaction to detection, and to compose a miniature total analytical system for automation. With the continuous development of new materials and technologies, the application of biochips will inevitably continue to expand from laboratory research applications to a wider range of clinical applications.