1. Laboratory preparation:
(1) Chemical laboratory: washing, drying, preservation in histoculture; preparation and dispensing of culture medium; autoclaving; handling of large-scale plant materials, as well as physiological and biochemical analyses are carried out in the chemical laboratory. The requirements are the same as those of a general chemistry laboratory.
(2) inoculation room (aseptic room): with ultra-clean bench or inoculation box, for sterile inoculation. Requirements for indoor smooth and smooth, flat and seamless ground to avoid dust accumulation. Indoor requirements for regular fumigation with formaldehyde or potassium permanganate sterilization, can also be used to irradiate ultraviolet light for more than 20 minutes.
(3) Culture room: the indoor requirements of clean, temperature control, lighting equipment and culture racks and other devices. Indoor temperature requires constant temperature, automatic temperature control equipment, generally require 25 ~ 27 ℃ or depending on the cultivation of plants. Light source to white fluorescent lamp is good.
In addition to the above necessary equipment, if possible, there can also be a cell laboratory and photography room.
2. Instrumentation:
(1) balance: balance accuracy of 0.1 grams of drug balance. The analytical balance with an accuracy of 0.001 grams and 0.0001 grams is used to weigh sucrose, agar, massive elements, trace elements and hormones and other medicines in the culture medium respectively.
(2) oven and thermostat: used for drying glassware and determine the dry weight of cultures.
(3) refrigerator: storage of various vitamins, hormones and culture medium mother liquor, preservation of experimental materials and materials for low temperature treatment.
(4) acidity meter: determine the pH of the culture medium.
(5) autoclave: autoclave sterilization of culture medium and glassware and other utensils.
In addition, it should be equipped with microscopes, microphotography, centrifuges, and suspension culture with a rotary bed, shaker, etc..
3. Glassware and utensils:
Commonly used glassware are various types of test tubes, triangular flasks, Petri dishes, measuring cylinders and beakers. Commonly used utensils can choose medical equipment or microbiological experiments used in a variety of tweezers, scissors, scalpels and dissecting needles.