What equipment does the PCR laboratory need?

PCR laboratory can be divided into three areas: sample preparation, nucleic acid diffusion and product analysis. The equipment used in each area is different. Let's give you a detailed introduction of what equipment is needed in each area of PCR laboratory.

Sample preparation area:

Biosafety cabinet: prevent the spread of harmful suspended particles and aerosols.

Sample low-temperature refrigerator: -20℃, -80℃ to store samples and DNA.

High-speed tabletop freezing centrifuge: collecting microbial cells, cell fragments and other sediments. Some samples need a low temperature environment because they are unstable at room temperature.

Water bath pot or metal bath: used for water bath temperature control during DNA hybridization.

Pipette: Accurate measurement of a specific volume of solution.

Ultraviolet lamp or disinfection vehicle: disinfection of space environment

Mixer: It needs to be mixed well before moving the sample book.

Clean workbench: operations involving bacteria must be completed under the ultra-clean workbench.

Low temperature shaker: used for shaking flask culture of Escherichia coli and yeast.

Magnetic stirrer: accelerate the dissolution of solid substances.

Nucleic acid diffusion region:

Gene amplifier: DNA/RNA amplification for gene detection.

Fluorescence quantitative PCR instrument: quantitative analysis of nucleic acid

Nucleic acid extractor: sample DNA/RNA extraction

Pipette: Accurate measurement of a specific volume of solution.

Ultraviolet lamp or disinfection vehicle: disinfection of space environment

Clean workbench: operations involving bacteria must be completed under the ultra-clean workbench.

Pure water device: used for PCR, DNA sequencing needs ultra-pure water.

Product analysis area:

Electrophoresis instrument: horizontal electrophoresis is used to detect nucleic acid DNA/RNA, vertical electrophoresis is used to detect protein, and transfer electrophoresis is used to transfer protein to membrane for weatern detection.

Gel imaging system/ultraviolet detector: used for observing and photographing nucleic acid agar electrophoresis and protein polyacrylamide; Uv analyzer is used for electrophoresis gel observation of nucleic acid after staining, and cannot be connected to a computer.

Electric furnace: electrophoresis agar gel is heated and melted.