This reagent is used to quantitatively determine the content of homocysteine in human serum in vitro.
test method
Cyclic enzyme method
Determination conditions: rate method, main wavelength: 340nm, secondary wavelength: 700nm.
operation sequence/order
Sample 13 microliter
Reagent1240μ l
Mix well and incubate at 37℃ 1 ~ 5 minutes.
Reagent 3 65 L.
Mix well, incubate at 37℃ for 2 minutes, continuously monitor 1-2 minutes, and calculate δ A/min.
Automatic biochemical analyzer has its own program parameter input method. The above basic parameters need to be combined with the program parameter input method of the automatic biochemical analyzer, and the reagents can be automatically determined with the supporting instruments after the computer parameters are input.
Quality control program
Select the quality control corresponding to the calibrator, and the measured value of the quality control should be within the specified range. If the result is out of range, please follow the following steps to find the reason:
1. Check whether the parameter settings and light sources are correct.
2. Check whether the cuvette and the sample suction needle are clean.
3. Check whether the water is polluted, and the growth of bacteria will lead to incorrect results.
4. Check the reaction temperature.
5. Check the validity of the kit.
Calculate Δ a measured value
Homocysteine concentration (μ mol/L) = standard concentration of homocysteine ×————
△ A standard
Reference value (reference range)
Serum: 3-15 μ mol/L.
It is suggested that each laboratory establish its own reference range.
Matters needing attention
1. It is only used for in vitro diagnosis. If the preservative contained in the reagent accidentally splashes on the skin, eyes and other human surfaces, it must be washed with clear water. If you eat by mistake, you need to go to the hospital for treatment.
2. If there is no filter with the required wavelength in the instrument, please choose a filter with similar wavelength.
3. Before blood collection, please try to avoid a high-protein diet, which may lead to an increase in homocysteine.
4. If the reagent becomes turbid or the blank absorbance value is less than 0.8, it cannot be used and should be discarded.
5.SAH will cause serious positive interference. However, the plasma of normal people contains SAH or SAH that cannot be detected below 1nmol/L, which will not cause interference. SAH pollution should be avoided during the test.
6. 3- azoadenosine in red blood cells can inhibit the key enzymes in the reaction system, thus inhibiting the production of HCY. Specimens containing 3- azoadenosine cannot be used in this product.
7. Patients receiving the following drugs will cause high levels of HCY detection: methotrexate, carbamazepine, phenytoin, NO, 6- azauridine, etc.
8. The waste liquid generated after reagent reaction and the packaging materials that are difficult to degrade after use should be collected and handed over to the local environmental waste treatment station for treatment.