Automatic biochemistry analyzer and medical equipment has been popularized to all levels of hospitals, and the fast and accurate results have provided great convenience for clinical diagnosis. However, due to the lack of corresponding understanding of the principle and performance of the instrument by each operator, the results of the automatic biochemical analyzer test results appear larger errors. The factors affecting the results of automatic biochemical analyzer are summarized as follows:
1, instrumental reasons
Biochemical analyzer water purifier technology in the light source lamps, transmittance windows and temperature incubation pool of water is not clean. Light source lamp over the use of the period of time is not replaced, transmittance window is not clean, warming pool of water with bacterial growth to produce floc floating material, will directly cause light scattering affect the colorimetric results.
The cleanliness of the reaction cup directly affects the accuracy of the analytical results.
Whether there are air bubbles in the reagent pipette and sample pipette, and whether the seal ring is aging. If you find bubbles in the pipette syringe to replace the sealing ring in time.
2 Reagents, calibrators, quality control products reason
Reagent kit selection: should see whether this reagent is compatible with the biochemistry instrument, such as samples, reagents, wavelengths, reaction temperatures, determination of time and methodology, etc., and secondly, to carry out a methodological evaluation before use. It is best to use a kit that meets the requirements of the Clinical Laboratory Center of the Ministry of Health, read the instruction manual carefully before use, and enter the parameters according to the parameter design principles of the instrument.
The reagents exceeded the expiration date or were improperly stored, which made the calibration wrong and caused systematic errors in the test results.
Cross-contamination of reagents: The reaction cups, dispensers and stirring needles of the majority of automated analyzers are not dedicated, and according to the reaction principle of the reagents, there is an interference phenomenon between the reagents.
Can cause:
①Reaction between the reagents;
②The effect of the residual reagent (immunoturbidimetric assay);
③Residual reagent and the reaction between the newly added samples;
④Residual reagent and the samples, reaction between the reagents. Therefore, when setting up the test items should be in accordance with the principle of reagent reaction to adjust the order of the test items.
3 Improper parameter settings
The main parameter settings include:
①Determination method, reaction type;
②Sample volume fraction;
③Primary and secondary wavelengths;
④Reaction time and temperature;
⑤Calculation factor;
⑥Linear range and absorbance of the upper and lower limits. lower limit of absorbance. If the above parameters are not set correctly, the results will be greatly affected.
4 Other reasons
4.1 The reason for the water treatment machine: the resistance value of the produced water exceeds the limit value so that the water quality is impure, the storage tank is not cleaned to breed microorganisms.
4.2 Sample: placed too long, hemolysis, celiac blood, jaundice, the sample contains reducing substances and glucose, etc..
4.3 Human factors: in the automated analysis of the control of all quality aspects of the human, before the analysis of the patient factors to consider, the whole process of specimen collection and transportation; the analysis of the performance of the instrument to consider, the working state, the working environment, reagents, parameter settings, indoor quality control, etc.; after the analysis of the results of the reliability of the results should be considered, and the patient's clinical symptoms whether or not to comply with the presence or absence of interfering factors.