There are several methods for the determination of nitric oxide:
1. Colorimetric method: the content of nitric oxide is indirectly determined by detecting the colored products produced after the reaction between nitric oxide and certain reagents. This method is simple to operate, but the sensitivity is low. Fluorescence method: to determine the content of nitric oxide by utilizing the fluorescence intensity emitted after combining nitric oxide with certain fluorescent dyes. This method is more sensitive, but requires special fluorescence detection equipment.
2, electrode method: through the measurement of nitric oxide in the electrode surface chemical reaction generated by the current or potential changes to determine the content of nitric oxide. This method is easy to operate, but is greatly affected by the performance of the electrode. Chemiluminescence method: Using the light signal generated by the reaction between nitric oxide and certain chemiluminescence reagents to determine the content of nitric oxide.
3, gas chromatography: by converting the nitric oxide in the sample into a measurable substance, and then quantitatively analyze it using a gas chromatograph. This method is highly accurate, but the operation is complicated and costly. Mass Spectrometry: By converting the nitric oxide in the sample into a measurable substance and then quantitatively analyzing it using a mass spectrometer.
Related Knowledge of Nitric Oxide
1. Nitric Oxide (NO) is a colorless, odorless, poisonous gas with the chemical formula NO. It exists widely in nature, such as nitrogen oxides in the atmosphere, and nitrogen oxides produced by lightning. Nitric oxide has important physiological functions in living organisms, such as regulating vascular tone, immune response, and nerve conduction.
2. Nitric oxide is widely used in medicine. It is an important drug carrier, which can regulate the contraction and diastole of vascular smooth muscle by changing the concentration of intracellular calcium ions, thus playing the role of dilating blood vessels and lowering blood pressure. In addition, nitric oxide can inhibit platelet aggregation and prevent thrombosis, which is important for the treatment of cardiovascular diseases.
3, however, nitric oxide also has a certain degree of toxicity. When its concentration is too high, it will have adverse effects on the human body, such as causing headache, nausea, breathing difficulties and other symptoms. Therefore, when using nitric oxide as a drug, the dosage and duration of use need to be strictly controlled.
4. In recent years, the application of nitric oxide in agriculture has also achieved remarkable results. It has been found that nitric oxide can be used as a plant growth regulator to promote plant growth and increase yield. For example, by spraying nitric oxide fertilizer, it can increase the yield of rice, wheat and other crops. In addition, nitric oxide can be used as a natural fungicide to control plant diseases.