1, due to the laser and fluorescence *** focus imaging and Pinhole application to prevent the focus of the interference outside the diffracted light and scattered light, *** focus microscope resolution is far from the ordinary fluorescence microscope can be, which is also one of the reasons why the international *** focus microscope is very popular.
2, *** focus microscope in the reflected light on the optical path plus a half-reflective half-lens, will have been through the lens of the reflected light folded in other directions, in its focus on a baffle with a pinhole, the hole is located in the focal point, behind the baffle is a photomultiplier tube. Detecting light before and after the focal point of the reflected light through this set of **** focus system, can not focus on the small hole, will be blocked by the baffle. So the photometer measures the intensity of the reflected light at the focal point.