High-pressure steam sterilizer as an example of how to use: Preparation: first of all, the inner sterilization barrel out, in the outer pot to add the appropriate amount of water, so that the water surface and the triangular shelf level is appropriate. In general, water should be added before each sterilization. Put back the inner sterilization bucket and load the items to be sterilized: Wrap the culture medium and empty glassware ready to be sterilized in kraft paper and load them into the inner suite of the pot. Add the lid: first check whether the sealing ring on the lid is good, and remove the dirt, and then insert the exhaust hose on the lid into the exhaust groove of the inner sterilizing drum, and then cover the lid tightly, and tighten the bolts on the lid evenly by diagonal, so that the bolts are loosely tightened uniformly, and do not make the leakage of air. There are also some not with bolts, but like the pressure cooker for home use, just align the lid with the slot of the pot, turn it clockwise and snap it into place. Heat the pot with an electric stove or gas (most of them are heated by electric heaters) and open the exhaust valve at the same time so that the cold air in the pot can be discharged after the water in the pot boils. Special attention should be paid here, the sterilization pot of cold air exclusion is extremely important. Because the expansion pressure of air is greater than the expansion pressure of water vapor, so when the water vapor contains air, at the same pressure, containing air steam temperature is lower than the temperature of saturated steam. Usually should wait for the pot of water boiling exhaust 3~5min, to be completely exhausted after the cold air, close the exhaust valve, so that the temperature inside the pot with the increase in steam pressure and gradually rise. Another way to exclude the cold air is: according to the above method to cover the pot tightly, heating the pot to produce steam. When the pressure gauge pointer reaches 33.78kPa, open the exhaust valve, the cold air out, when the pressure gauge pointer down. When the pointer drops to zero, the cold air exhaust, close the exhaust valve. When the pressure in the pot rises to the required pressure, control the heat source, maintain pressure to the required time. After reaching the required time for sterilization, cut off the power supply or turn off the gas. Let the temperature inside the sterilizing pot drop naturally, and when the pressure of the pressure gauge drops to 0, open the exhaust valve, loosen the bolt, open the lid of the pot, and take out the sterilized items after 10min. Be careful, if the pressure does not drop to 0 to open the exhaust valve, will be due to the sudden drop in pressure inside the pot, so that the liquid in the container due to the internal and external pressure imbalance and rushed out of the mouth of the flask or test tube, resulting in cotton plugs stained media and contamination occurs.
Suzhou Haisiyuan
Automatic Autoclave Sterilizer